关键词: 墨兰, 原球茎, 根状茎, 根毛, 芽分化

Abstract: The aseptic seedling results of Cymbidium scinense were observed that when they were cultured on the media supplemented without any cytokinin, seeds can sprout but only protocorm and rhizome came into being, and they cannot differentiate further into seedlings. Only on the MS or Knudson C media containing different hormones can they induce shoot initiation. The medium added with 0.5~1.0mg/L 6-BA and 0.1mg/L NAA gave the best results. The MS medium added with 2.0mg/L BA and 0.4mg/L NAA can accelerate greatly shoot differentiation rate, when rhizome was transferred onto the liquid proliferation medium added with the same hormones and cultured at the stage of vibration. The medium added with 0.5% activated carbon had obvious synergy effect for shoot differentiation. MS medium added with 0.2mg/L NAA gave the best result in seedling rooting.

Key words: Cymbidium scinense, protocorm, rhizome, root hair, bud differentiation