关键词: HLC, 2, 4-二硝基氯苯, 氨基酸, 柱前衍生

Abstract: A method for separation and quantification of primary and secondary amino acids by pre-column derivation with 1-fluoro-2,4-dinitrobenze and reversed-phase HPLC is described. 15 amino acids can be separated thoroughly with a linear gradient elution with acetate buffer and acetonitrile solution. The products of derivation can be kept at room temperature for over two months with little change in their contents. The lowest content that can be determined was below 5 pmol. The method may be applied to the determination of amino acid composition of foods and drugs, and so on.

Key words: HPLC, amino acid, 1-fluoro-2,4-dinitrobenze, derivation