关键词: 花生叶肉细胞, 原生质体, 酶解, 丙二醛, 过氧化物酶, 过氧化氢酶, 超氧化物歧化酶, 抗坏血酸

Abstract: Malondialdehyde(MDA) accumulated in protoplast of mesophyll cell of Arachis hypagaea during the cell wall digestion in enzyme solution. Added with ascorbic acid (ASA), the content of MDA decreased. At the initial stage of enzyme digestion, the activities of peroxidase (POD) and catalase (CAT) increased without adding ASA and after a long time of enzyme digestion, the activity of POD decreased. While ASA added into enzyme solution, the activity of superoxide disumutase (SOD) increased significantly. These results showed that membrane were damaged during the protoplasts isolation; cells had a protection mechanism to respond to it and adding ASA could reduce the membrane damage during protoplasts isolation.

Key words: mesophyll cell of Arachis hypogaea, protoplast, enzyme digestion, MDA, POD, CAT, SOD, ASA