关键词: 杧果, LEAFY基因, PCR, 分子克隆, 序列分析

Abstract: Analyzing a conserved sequence of the LEAFY (LFY) gene, which plays an important role in floral development, a pair of 23 bp primers was used to amplify an 822 bp fragment by polymerase chain (PCR) with genomic DNA of mango. The fragment was cloned into pGEM-T Easy vector, and then sequenced. A fragment in the 3'-end of LFY homologous gene named miLFY gene was obtained. The result of sequence analysis indicated that there was an intron of 415 bp in the fragment, and the exons encoded 135 amino acids. The miLFY gene has been registered in GenBank with the accession number AY189684. After the deduced amino acid sequence of the fragment was submitted to GenBank and blast with LFY homologous gene of other plants, it was found that the homology reached 74%~97%. This result suggested that miLFY gene might have the same function as other LFY homologous gene.

Key words: mango, LEAFY gene, PCR, molecular cloning, sequence analysis