不同低温需求量砂梨休眠相关基因PpMADS13的特征及表达模式

doi:10.3969/j.issn.1009-7791.2016.01.009

亚热带植物科学 ›› 2016, Vol. 45 ›› Issue (01): 42-47.DOI: 10.3969/j.issn.1009-7791.2016.01.009

• 植物生理生化与分子生物学 • 上一篇下一篇

不同低温需求量砂梨休眠相关基因PpMADS13的特征及表达模式

李亮,刘杭,张桂池,吴少华,李永裕

(福建农林大学园艺学院，园艺植物天然产物研究所，福建福州 350002)

收稿日期:2015-12-08 修回日期:2016-04-17 出版日期:2016-03-10 发布日期:2016-03-10
通讯作者: 吴少华;李永裕
作者简介:李亮，硕士研究生，从事果树分子生物学研究。
基金资助:
国家自然科学基金(31501694)；高等学校博士学科点专项科研基金(20113515110011)

Characteristics and Expression of Dormancy-Associated MADS-Box PpMADS13 in Different Chilling Requirement Pear Cultivars

LI Liang,LIU Hang,ZHANG Gui-chi,WU Shao-hua,LI Yong-yu

(College of Horticulture, Fujian Agriculture and Forestry University; Institute of Natural Products of Horticultural Plants, Fuzhou 350002, Fujian China)

Received:2015-12-08 Revised:2016-04-17 Online:2016-03-10 Published:2016-03-10
Contact: WU Shao-hua; LI Yong-yu

摘要/Abstract

摘要： 为探究福建主栽的不同低温需求量砂梨Pyrus pyrifolia品种中休眠相关基因PpMADS13-1和PpMADS13-3序列及表达特征，以‘黄花’、‘蜜雪’两品种休眠时期的花芽为材料，克隆PpMADS13-1和PpMADS13-3基因，利用荧光定量PCR技术分析基因在休眠时期的表达变化情况。结果显示，PpMADS13-1序列在砂梨两品种间较保守，而PpMADS13-3序列在两品种间保守性较差；‘黄花’梨PpMADS13-1hh和PpMADS13-3hh在休眠期间随着休眠的解除下调表达，‘蜜雪’梨PpMADS13-1mx和PpMADS13-3mx在休眠期间呈现“M”型表达趋势。PpMADS13-1和PpMADS13-3均对砂梨的花芽休眠解除起到调控作用，在不同低温需求量品种间PpMADS13-1和PpMADS13-3的休眠调控模式有所不同。

关键词: 砂梨, 休眠相关MADS-box, 表达

Abstract: The buds from ‘Huanghua’ and ‘Mixue’, two cultivars of Pyrus pyrifolia, were used as the experiment materials to explore the characteristics and expression of dormancy-associated MADS-box (DAM) genes PpMADS13-1 and PpMADS13-3 in different chilling requirement pear cultivars from Fujian. PpMADS13 genes were cloned and used the QRT-PCR to analysis their expression changes during the dormancy period. The results showed that PpMADS13-1 was conservative in low chilling requirement pears. The conservatism of PpMADS13-3 was not so high in different chilling requirement pears. The PpMADS13-1hh and PpMADS13-3hh in ‘Huanghua’ were gradual down-regulation with dormancy release. The PpMADS13-1mx and PpMADS13-3mx in ‘Mixue’ presented a trend of ‘M’ type during the dormancy. The result indicated that both PpMADS13-1 and PpMADS13-3 work in dormancy release, but their regulation model were different between two pear cultivars.

Key words: Pyrus pyrifolia, dormancy-associated MADS-box, expression

中图分类号:

S661.2

李亮,刘杭,张桂池,吴少华,李永裕. 不同低温需求量砂梨休眠相关基因PpMADS13的特征及表达模式[J]. 亚热带植物科学, 2016, 45(01): 42-47.

LI Liang,LIU Hang,ZHANG Gui-chi,WU Shao-hua,LI Yong-yu. Characteristics and Expression of Dormancy-Associated MADS-Box PpMADS13 in Different Chilling Requirement Pear Cultivars[J]. Subtropical Plant Science, 2016, 45(01): 42-47.

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不同低温需求量砂梨休眠相关基因PpMADS13的特征及表达模式

Characteristics and Expression of Dormancy-Associated MADS-Box PpMADS13 in Different Chilling Requirement Pear Cultivars

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