基于流式细胞术测定狸尾豆和蝙蝠草基因组大小

doi:10.3969/j.issn.1009-7791.2024.06.002

亚热带植物科学 ›› 2024, Vol. 53 ›› Issue (6): 503-511.DOI: 10.3969/j.issn.1009-7791.2024.06.002

基于流式细胞术测定狸尾豆和蝙蝠草基因组大小

高颖捷¹，缪佳²，黄翔^3,4，赵雪利^1*

(1. 西南林业大学西南地区生物多样性保育国家林业和草原局重点实验室，云南昆明 650224；2. 云南省热带作物科学研究所，云南景洪 666100；3. 福建农林大学林学院，福建福州 350002；4. 3S技术与资源优化利用福建省高校重点实验室，福建福州 350002)

收稿日期:2024-08-08 接受日期:2024-08-28 出版日期:2024-12-31 发布日期:2025-03-20
通讯作者: 赵雪利
基金资助:
国家自然科学基金项目(31800170)

Genome Size Estimation of Uraria lagopodioides and Christia vespertilionis (Fabaceae) by Flow Cytometry

GAO Ying-jie¹, MIAO Jia², HUANG Xiang^3,4, ZHAO Xue-li^1*

(1. Key Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Southwest China, Southwest Forestry University, Kunming 650224, Yunnan China; 2. Yunnan Institute of Tropical Crops, Jinghong 666100, Yunnan China; 3. College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian China; 4. University Key Lab for Geomatics Technology and Optimize Resources Utilization in Fujian Province, Fuzhou 350002, Fujian China)

Received:2024-08-08 Accepted:2024-08-28 Online:2024-12-31 Published:2025-03-20
Contact: ZHAO Xue-li

摘要/Abstract

摘要： 为测定豆科药用植物狸尾豆Uraria lagopodioides和蝙蝠草Christia vespertilionis的基因组大小，并探究其适合的实验体系，本研究筛选了5种细胞核解离缓冲液以及2种细胞核悬液制备方法，以玉米Zea mays为内标植物，运用流式细胞术对两种植物的基因组大小进行测定。结果显示，适合狸尾豆和蝙蝠草流式细胞术的细胞核解离缓冲液为MGB；40 μm孔径滤网过滤3次，或1000 r·min^–1离心是适合的细胞核悬浮液制备条件；狸尾豆和蝙蝠草基因组大小分别为0.69 Gb和0.64 Gb。该研究结果为狸尾豆和蝙蝠草基因组学、遗传进化、资源开发利用研究提供参考。

关键词: 流式细胞术, 狸尾豆属, 蝙蝠草属, 核DNA含量, 豆科

Abstract: This study aimed to determine the genome size of medicinal plants Uraria lagopodioides and Christia vespertilionis (Fabaceae), and to explore the appropriate experimental conditions. We screened five nuclear dissociation buffer and two methods for preparing nuclear suspensions, and then we estimated the genome size of both species by flow cytometry using the most appropriate experimental conditions with Zea mays as an internal standard plant. The results revealed that MGB was a suitable nuclear dissociation buffer for flow cytometry of both plants, and filtering through the 40 μm mesh for three times or centrifugation at 1000 r·min^–1 was the appropriate method for preparing nuclear suspensions. The genome size of U. lagopodioides and C. vespertilionis were estimated to be 0.69 Gb and 0.64 Gb, respectively. Genome size estimation and the exploration of suitable experimental conditions for flow cytometry in this study can provide reference for the genomics, genetic evolution, and resource development of U. lagopodioides and C. vespertilionis.

Key words: flow cytometry, Uraria, Christia, nuclear DNA content, Fabaceae

中图分类号:

Q943.2

高颖捷, 缪佳, 黄翔, 赵雪利. 基于流式细胞术测定狸尾豆和蝙蝠草基因组大小[J]. 亚热带植物科学, 2024, 53(6): 503-511.

GAO Ying-jie, MIAO Jia, HUANG Xiang, ZHAO Xue-li. Genome Size Estimation of Uraria lagopodioides and Christia vespertilionis (Fabaceae) by Flow Cytometry[J]. Subtropical Plant Science, 2024, 53(6): 503-511.

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基于流式细胞术测定狸尾豆和蝙蝠草基因组大小

Genome Size Estimation of Uraria lagopodioides and Christia vespertilionis (Fabaceae) by Flow Cytometry

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