关键词: 花生根瘤, 类菌体, H₂氧化体系

Abstract: An investigation was conducted to identify electron transport carriers that participated the oxidation of H₂ in bacteroids of Rhizobium arachis.H₂-reduced mi-nus O₂-oxidized absorption difference spectra in bacteroids showed peaks at 424,500,520,550,560,585 and 595 nm,indicating the involvement of c-,b-,a and o-types of cytochromes,The absorption peaks at 520,550,560 nm were diminished a lot in the pres-ence of CN^- This showed that some of c- and b-type cytochromes were CN^- sensitive and therefore might play a role as terminal oxidases in H₂ metabolism. H₂-uptake was in-hibited by antimycin A,HONO,CN^-,N₃^- and DBMIB,not by rotenose,Our resultsindicate that ubiquinone and some types of cytochromes are involved in the oxyhydro-gen reaction which is a complex,branched transport system.

Key words: Peanut nodules, Bacteroid, H₂-oxidizing system