关键词: 大花蕙兰, 类原球茎, 形态发生, 组织学

Abstract: Anatomy study on the protocorm-like bodies formation and development of Cymbidium hybridium was conducted. The results showed that callus formed firstly from shoot tip of explant, then differentiated into protocorm-like bodies. In the process of protocorm-like bodies proliferation, variegated forms of pre-protocorms formed around one protocorm-like body which developed into a plantlet at the same time. As for the controversial question of protocorm-like bodies derivation, our results showed that the origin of protocorm-like bodies was a specific regeneration process that have both characteristics of the pathways of somatic embryogenesis and organogenesis; the exact regeneration pathway differed according to genotypes and culture conditions.

Key words: Cymbidium hybridium, protocorm-like bodies, morphogenesis, histology