关键词: 大花蕙兰, 组织培养, 丛生芽增殖, 丛生芽生根

Abstract: To set up a new tissue culture system with the buds of Cymbidium hybridum as tissue explants, N6, B5, CC and MS as basic medium, orthogonal experiment design was carried out with macroelement, microelement and organic matter as three different influencing factors. Results showed that the best medium combination for developing C. hybridum was B5 macroelement + CC microelement + MS organic + sucrose 30 g·L-1 + agar 7 g·L-1 + activated carbon 0.5 g·L-1 (pH 5.8—6.0), the best medium hormone concentration combination for multiple bud proliferation was 6-BA 4.0 mg·L-1+ NAA 0.2 mg·L-1，the best hormone concentration for induce in vitro shoot rooting was NAA 1.0 mg·L-1.

Key words: Cymbidium hybridum, tissue culture, proliferating of multiple buds, rooting of multiple buds