关键词: 脱落酸, 绿豆下胚轴, 细胞周期时相, 生长素, 不定根

Abstract: Pre-treatment of germinating seeds of mung bean with 10^-4 mol/L of abscisic acid (ABA) significantly promoted adventitious root formation from rooting zone of hypocotyl cuttings of mung bean.Root number and rooting zone from each 4-day-old cutting were increased by 174% and 45%, respectively, when compared with those of the control cuttings.The enzyme activities of tryptophan aminotransferase, indole pyruvate decarboxylase and indole acetaldehyde dehydrogenase (three enzymes involved in indole acetic acid biosynthetic pathway) in rooting zone of 4-day-old hypocotyl cuttings, were increased by 49.1%, 90.6% and 105.4%, respectively, resulting in a 63.9% increase of IAA content, when compared with those of the control cuttings.Cell number in S-phase of a cell cycle in the rooting zone of 2-day-old cuttings was increased by 13% with the treatment of 10-4mol/L ABA.These results suggested that ABA was possibly promoted adventitious root formation by stimulation of DNA and IAA synthesis.

Key words: abscisic acid, hypocotyl cuttings of mung bean, cell cycle phase, auxin, adventitious root