亚热带植物科学 ›› 2013, Vol. 42 ›› Issue (01): 27-30.

• 研究报告 • 上一篇    下一篇

野生碧玉兰组培快繁技术

王亚沉;王玉英;周慧恒;李枝林   

  1. 云南农业大学园林园艺学院花卉研究所;云南农业大学协同创新中心;
  • 收稿日期:2013-02-27 修回日期:2013-02-27 出版日期:2013-03-10 发布日期:2013-03-10
  • 通讯作者: 李枝林
  • 基金资助:

    国家自然科学基金项目

Research on Techniques of Tissue Culture and Rapid Propagation of Wild Cymbidium lowianum

  • Received:2013-02-27 Revised:2013-02-27 Online:2013-03-10 Published:2013-03-10

摘要: 以云南野生碧玉兰(Cymbidium lowianum)种子无菌萌发的试管苗为试验材料,进行其丛生芽诱导增殖、生根的组培快繁技术体系研究。结果表明:适宜的增殖培养基配方为1/2MS+6-BA 2.0 mg/L+KT 0.5 mg/L+NAA 0.2 mg/L,增殖率可达300%;生根培养基的配方为1/2MS+NAA 1.5 mg/L+6-BA 0.3 mg/L,其根数多,健壮。

关键词: 碧玉兰, 组培, 增殖, 生根

Abstract: With the in vitro germination plantlet of wild Cymbidium lowianum in Yunnan as test material, a series of tissue culture and rapid propagation system research including induced proliferation and rooting for its multiple shoots was carried out. The results showed that the suitable multiplication medium was 1/2MS + 6-BA 2.0 mg/L + KT 0.5 mg/L + NAA 0.2 mg/L, with the proliferation rate of 300%, and the rooting medium was 1/2MS + NAA 1.5 mg/L + 6-BA 0.3 mg/L, then the roots healthy and strong.

Key words: Cymbidium lowianum, tissue culture, proliferation, rooting

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