绿色木霉在啤酒糟基质上产纤维素酶条件的优化

亚热带植物科学 ›› 2007, Vol. 36 ›› Issue (03): 23-26.

绿色木霉在啤酒糟基质上产纤维素酶条件的优化

陈健旋,陈莲

漳州职业技术学院, 福建漳州 363000

收稿日期:2006-12-14 出版日期:2007-09-10 发布日期:2007-09-10
作者简介:陈健旋(1965-),女,福建漳州人,副教授,从事微生物发酵技术研究。
基金资助:
漳州职业技术学院科研项目(ZZY0625)

Studies on the Optimum Condition of Trichoderma viride Persoon ex fries Fermentation to Produce Cellulase

CHEN Jian-xuan, CHEN Lian

Zhangzhou Vocational and Technical College, Zhangzhou 363000, Fujian China

Received:2006-12-14 Online:2007-09-10 Published:2007-09-10

摘要/Abstract

摘要： 对绿色木霉接种到啤酒糟固态发酵产纤维素酶的培养基和培养条件进行优化,考察发酵物料起始含水量、发酵时间、起始pH值等发酵条件,以及啤酒糟培养基中添加麸皮、氮源种类对产酶的影响。结果表明,以啤酒糟为发酵基质接种绿色木霉生产纤维素酶是可行的。经单因素和正交试验获得最适固态发酵的培养条件为:起始pH 5~6,培养温度28~30℃,发酵4 d;最佳发酵培养基组合为:麸皮比例30%,培养基起始含水量50%,(NH₄)₂SO₄添加量为2.0%~2.5%。

关键词: 绿色木霉, 啤酒糟, 纤维素酶, 优化条件

Abstract: Solid-state fermentation process was carried out to convert brewer's grains to produce cellulase by Trichoderma viride Persoon ex fries.Such factors including initial moisture, fermentation time, initial pH value, the amounts of wheat bran and nitrogen source types were studied in this experiment.An optimum medium ingredient was obtained by primary test of single factor and orthogonal design experiment.The results were as follows.The best fermentation conditions were initial pH 5~6, fermentation temperature 28~30℃ and fermentation time 4d, and the best medium composition was wheat bran 30%, water contain 50% at least and (NH₄)₂SO₄ 2.5%.

Key words: Trichoderma viride, brewers, cellulase, optimum, condition

中图分类号:

TQ925

陈健旋,陈莲. 绿色木霉在啤酒糟基质上产纤维素酶条件的优化[J]. 亚热带植物科学, 2007, 36(03): 23-26.

CHEN Jian-xuan, CHEN Lian. Studies on the Optimum Condition of Trichoderma viride Persoon ex fries Fermentation to Produce Cellulase[J]. Subtropical Plant Science, 2007, 36(03): 23-26.

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