亚热带植物科学 ›› 2006, Vol. 35 ›› Issue (04): 35-38.

• 研究报告 • 上一篇    下一篇

南方碱蓬根茎叶总黄酮提取与含量测定

黄晓昆,朱加元,黄晓冬,缪翠艳,陈洁芳   

  1. 泉州师范学院 教育科学学院, 福建 泉州 362000
  • 收稿日期:2006-06-27 出版日期:2006-11-10 发布日期:2006-11-10
  • 作者简介:黄晓昆(1973-),女,福建泉州人,讲师,硕士,从事植物资源化学研究。

Extraction and Determination of Total Flavonoids from Root, Stem and Leaf of Suaeda australis

HUANG Xiao-kun, ZHU Jia-yuan, HUANG Xiao-dong, MIU Cui-yan, CHEN Jie-fang   

  1. College of Educational Science, Quanzhou Normal University, Quanzhou 362000, Fujian China
  • Received:2006-06-27 Online:2006-11-10 Published:2006-11-10

摘要: 以不同浓度的酒精、料液比、提取温度及提取时间等条件,对南方碱蓬根、茎、叶总黄酮提取进行正交设计实验,并用聚酰胺吸附—硝酸铝显色法测定其总黄酮含量。结果表明,总黄酮的优化提取条件,根为50倍原料重的45%乙醇溶液在80℃条件下提取30 min,含量为(1.50±0.20)%;茎为30倍原料重的60%乙醇溶液在80℃条件下提取50 min,含量(0.94±0.24)%;叶为30倍原料重的75%乙醇溶液在80℃条件下提取30 min,含量为(6.35±0.45)%。

关键词: 南方碱蓬, 根, 茎, 叶, 总黄酮, 含量测定

Abstract: The effects on the flavonoids content in roots, stem and leaves of Suaeda australis of different concentration ethanol, ratio of material to liquid, extraction temperature and time were studied by the orthogonal experiment.Extracted by the optimum extraction conditions, purified by polycaprolactan, produced color with Al(NO3)3, the flavonoids content was determined.Results showed that the optimum conditions of extraction were as follows:from root, extracted by 50:1, 45% ethanol at 80℃ for 30min;from stem, extracted by 30:1, 60% ethanol at 80℃ for 50min;from leaf, extracted by 30:1, 75% ethanol at 80℃ for 30min.The flavonoids content in root, stem and leaf of Suaeda australis was 1.50%, 0.94%, 6.35%, respectively.

Key words: Suaeda australis, root, stem, leaf, total flavonoid, determination

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