亚热带植物科学 ›› 2005, Vol. 34 ›› Issue (01): 60-62.

• 研究报告 • 上一篇    下一篇

鱼腥草组织培养研究

龚伟,胡庭兴,宫渊波,张健,王景燕   

  1. 四川农业大学 生态林业工程省级重点实验室, 四川 雅安 625014
  • 收稿日期:2004-09-02 出版日期:2005-03-10 发布日期:2005-03-10
  • 作者简介:龚伟(1980-),男,四川崇州人,博士研究生,从事生态林业工程、生物技术应用研究。
  • 基金资助:

    四川省森林培育重点学科建设项目资助

Tissue Culture of Houttuynia cordata

GONG Wei, HU Ting-xing, GONG Yuan-bo, ZHANG Jian, WANG Jing-yan   

  1. Sichuan Provincial Key Laboratory of Ecological Forestry Engineering, Sichuan Agricultural University, Ya'an 625014, Sichuan China
  • Received:2004-09-02 Online:2005-03-10 Published:2005-03-10

摘要: 以鱼腥草带侧芽茎段为外植体进行组培快繁研究。结果表明,初代培养以MS+6-BA1.0mg/L+NAA0.05mg/L为最佳,30d侧芽诱导率可达66.7%;MS+6-BA 2.0 mg/L+2,4-D 0.2mg/L+NAA 0.2mg/L对芽苗的继代增殖效果最好,40d芽苗的增殖倍数可达7.4,且芽苗较高;以MS+6-BA3.0mg/L+2,4-D 0.5mg/L+NAA0.1mg/L对芽苗的继代增重效果最好,40d芽苗鲜重为7.358g;以沙和蛭石(1:1)为基质瓶外生根效果最好,30d芽苗生根率可达84%。

关键词: 鱼腥草, 侧芽培养, 生物量, 瓶外生根

Abstract: Taking stem segments with lateral buds as explants, the tissue culture of Houttuynia cordata were studied. The results showed that MS+6-BA 1.0mg/L+NAA 0.05mg/L was best for bud induction and the induction rate could reach 66.7% when cultured for 30d; MS+6-BA 2.0mg/L+2,4-D 0.2mg/L+NAA 0.2mg/L gave the best result for subculture of shoots and the multiplication rate could reach 7.4 times in 40d; MS+6-BA 3.0mg/L+2,4-D 0.5mg/L+NAA 0.1mg/L was best for increasing the weight of shoots and the fresh weight of shoots gain 7.358g in 40d; sand and vermiculite(1:1) was suitable for ex vitro shoots rooting and the rooting rate could reach 84%.

Key words: Houttuynia cordata, lateral bud culture, biomass, ex vitro rooting

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