关键词: 铁炮百合, 不定芽, 组织细胞学

Abstract: Sterilized leaf segments of lilium longiflorum were cultured for callus inducement and the results showed that MS+6-BA 2.0mg/L+NAA 0.2mg/L was the best for adventitious buds. The leaf segments were dissected at regular intervals to observe the initial places of dedifferentiation and histological changes during induced period. The results shows that the dedifferentiation initiates from wounded cells; the dedifferentiated cells form meristematic packed cells which were developed to be callus; the callus dedifferentiates and the adventitious buds form. The buds mainly develop from second to seventh layer cells of meristematic packed cells.

Key words: Lilium longiflorum, adventitious buds, cytohistology