关键词: 海边月见草, 溶剂萃取, 毛细管气相色谱法, 脂肪酸

Abstract: Oenothera littaralis oil was extracted from its seeds by three different solvents (ether, petroleum ether or chloroform-methanol).Fatty acids in the oil were transferred to methyl esters with BF₃-MeoH by means of rapid saponification and esterification, and then subjected to analyze by capillary GC. The quantitative determination was carried out by external standard method. The results showed that the contents of linoleic acid and γ-linolenic acid in the seed oil by three solvents had no significant difference, but petroleum ether was more suitable than the other two solvents if considering the remains of solvents. Furthermore, the components of γ-linolenic acid was satisfactorily separated from the other fatty acids, especially linoleic with these esterification methods by GC.

Key words: Oenothera littaralis, solvent extraction, capillary GC, fatty acids