›› 2011, Vol. 40 ›› Issue (02): 34-38.DOI: 10.3969/j.issn.1009-7791.2011.02.011

• 研究报告 • 上一篇    下一篇

‘巴斗杏’组培快繁体系建立与耐盐植株筛选

杜雪玲,余如刚,宋运贤,张振霞   

  1. (1.淮北师范大学 生命科学学院 资源植物学安徽省重点实验室,安徽 淮北235000;2.韩山师范学院 生物系,广东 潮州 521041)
  • 收稿日期:2010-12-27 修回日期:2010-12-27 出版日期:2011-06-10 发布日期:2011-06-10
  • 通讯作者: 杜雪玲
  • 作者简介:杜雪玲(1976-),女,河南扶沟人,硕士,讲师,从事植物细胞工程研究。
  • 基金资助:
    安徽省高校自然科学研究项目(KJ2008B230)

Establishment of Tissue Culture and Rapid Propagation System of Armeniaca vulgaris ‘Badou’and Selection of Salt-tolerant Plant

DU Xue-ling,YU Ru-gang,SONG Yun-xian,ZHANG Zhen-xia   

  1. (1.Anhui Key Laboratory of Plant Resources and Biology, College of Life Science, Huaibei Normal University, Huaibei 235000, Anhui China; 2.Department of Biology, Hanshan Normal University, Chaozhou 521041, Guangdong China)
  • Received:2010-12-27 Revised:2010-12-27 Online:2011-06-10 Published:2011-06-10
  • Contact: DU Xue-ling

摘要: 以淮北黄里‘巴斗杏’茎段为外植体,MS为基本培养基,通过茎段诱导植株再生及进行耐盐筛选。结果表明:采用4、5月‘巴斗杏’茎段用0.1%升汞灭菌8 min较适宜;在含有1.0 mg/L 6-BA和0.1 mg/L IBA的MS培养基上茎段增殖较快,生长旺盛;较理想的生根培养基为MS + NAA 0.1 mg/L + IBA 0.2 mg/L,生根率达46.3%;‘巴斗杏’组培苗进行耐盐筛选的适宜盐浓度为0.4%~0.8%,筛选植株与对照相比差异显著。

关键词: ‘巴斗杏’, 腋芽, 增殖, 生根, 耐盐筛选

Abstract: The stem of Armeniaca vulgaris ‘Badou’ was used as explants and MS as basic substrate to induce plant regeneration and select salt-tolerant plant. The results showed that the stem of ‘Badou’ in April and May sterilized by 0.1% mercury for 8min was suitable, the stem in MS culture medium with 1.0mg/L 6-BA and 0.1 mg/L IBA proliferated quickly and grew rushingly, the perfect rooting medium was MS+0.1 mg/L NAA + 0.2 mg/L IBA, with its rooting rate of 46.3%, and the optimum salt concentration in selection of salt-tolerant tissue culture seedlings was 0.4~0.8, there were also significant difference between plants selected and their contrast.

Key words: Armeniaca vulgaris ‘Badou’, axillary buds, proliferation, rooting, selection of salt-tolerant

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