亚热带植物科学 ›› 2023, Vol. 52 ›› Issue (5): 408-412.DOI: 10.3969/j.issn.1009-7791.2023.05.006

• 植物生理生化与分子生物学 • 上一篇    下一篇

铁甲秋海棠花粉离体萌发研究

吕燕玲*   

  1. (厦门市园林植物园,福建 厦门 361003)
  • 收稿日期:2023-07-17 接受日期:2023-08-17 出版日期:2023-10-30 发布日期:2024-01-19
  • 通讯作者: 吕燕玲
  • 基金资助:
    厦门市社会发展领域指导性项目(3502Z20234ZD4001)

In vitro Germination of Begonia masoniana Pollen

LÜ Yan-ling*   

  1. (Xiamen Botanical Garden, Xiamen 361003, Fujian China)
  • Received:2023-07-17 Accepted:2023-08-17 Online:2023-10-30 Published:2024-01-19
  • Contact: Lü Yan-ling

摘要: 为探究铁甲秋海棠Begonia masoniana花粉离体萌发适宜条件,采用液体培养法,通过单因素试验考察蔗糖、硼酸、PEG-4000、氯化钙浓度以及不同培养时间对花粉管生长的影响。结果表明,铁甲秋海棠花粉离体萌发的最适培养基为5%蔗糖+ 0.01%硼酸+ 5%PEG-4000 + 0.003%氯化钙;最佳观察时间为120 min。利用该培养基进行培养,铁甲秋海棠花粉萌发率达90.86%,花粉管长度达511.51 μm。该研究结果可为同属其他种类秋海棠的花粉研究及育种提供参考。

关键词: 铁甲秋海棠, 花粉萌发, 花粉管生长, 离体培养

Abstract: To explore the suitable conditions for in vitro germination of Begonia masoniana pollen, a liquid culture method was used to investigate the effects of sucrose, boric acid, PEG-4000, calcium chloride concentration, and different cultivation times on pollen tube growth through single factor experiments. The results showed that the optimal medium for pollen germination was sucrose 5%, boric acid 0.01%, PEG-4000 5%, calcium chloride 0.003%. The optimal observation time was 120 min. The pollen germination rate of B. masoniana was 90.86%. The length of the pollen tube was 511.51μm. Our results could provide reference for pollen research and breeding of other begonias.

Key words: Begonia masoniana, pollen germination, pollen tube growth, culture in vitro

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