转基因大豆GTS40-3-2环介导等温扩增检测方法的建立

doi:10.3969/j.issn.1009-7791.2015.03.007

›› 2015, Vol. 44 ›› Issue (03): 213-217.DOI: 10.3969/j.issn.1009-7791.2015.03.007

• 植物生理生化与分子生物学 • 上一篇下一篇

转基因大豆GTS40-3-2环介导等温扩增检测方法的建立

杨丽霞,邱华丽,彭新凯,宋涛平

（长沙市食品质量安全监督检测中心，湖南长沙 410013）

收稿日期:2015-05-17 修回日期:2015-11-26 出版日期:2015-09-10 发布日期:2015-09-10
通讯作者: 杨丽霞
作者简介:杨丽霞，博士，高级工程师，从事食品安全分子生物学研究。
基金资助:
湖南省质量技术监督局科研项目(2013KJJH11)

Establishment of Loop-mediated Isothermal Amplification Method for Detection of Transgenic Soybean GTS40-3-2 Strain

YANG Li-xia,QIU Hua-li,PENG Xin-kai,SONG Tao-ping

(Changsha Center of Supervision & Inspection on Food Quality Safety, Changsha 410013, Hunan China)

Received:2015-05-17 Revised:2015-11-26 Online:2015-09-10 Published:2015-09-10
Contact: YANG Li-xia

摘要/Abstract

摘要： 采用转基因大豆GTS40-3-2品系特异性基因LAMP引物，建立2种转基因大豆GTS40-3-2品系特异性基因等温扩增方法：(1)实时荧光环介导等温扩增方法；(2)反应前加入染料羟基萘酚蓝(HNB)作为LAMP扩增的指示剂，根据反应后HNB的颜色变化进行判定。对两种方法进行灵敏度、特异性测试，结果表明，两种方法皆能快速、特异性检测出转基因大豆GTS40-3-2品系成分，定性检测限为0.1%。基于颜色判定的LAMP检测方法对设备要求更简单，更适用于日常检验及监管部门进行转基因成分的快速检测。

关键词: 转基因大豆GTS40-3-2品系, 环介导等温扩增, 可视化检测

Abstract: According to the specific loop-mediated isothermal amplification (LAMP) primers, two specific LAMP methods for the detection of the transgenic soybean GTS40-3-2 strain were established: (1) real-time fluorescence loop-mediated isothermal amplification (RT-LAMP); (2) LAMP with hydroxy naphthol blue (HNB) dye as indicator. The specificity and sensitivity of these two methods were evaluated. The results indicated that the transgenic soybean GTS40-3-2 could be distinguished from several GM events and non-GM plants with limitation of dection of 0.1%. The LAMP assay with HNB dye requires only simple laboratory equipment and it is suitable for daily inspection and supervision departments for rapid detection of genetically modified ingredients.

Key words: transgenic soybean GTS40-3-2 strain, loop-mediated isothermal amplification, visual detection

中图分类号:

S565.1

杨丽霞,邱华丽,彭新凯,宋涛平. 转基因大豆GTS40-3-2环介导等温扩增检测方法的建立[J]. , 2015, 44(03): 213-217.

YANG Li-xia,QIU Hua-li,PENG Xin-kai,SONG Tao-ping. Establishment of Loop-mediated Isothermal Amplification Method for Detection of Transgenic Soybean GTS40-3-2 Strain[J]. , 2015, 44(03): 213-217.

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转基因大豆GTS40-3-2环介导等温扩增检测方法的建立

Establishment of Loop-mediated Isothermal Amplification Method for Detection of Transgenic Soybean GTS40-3-2 Strain

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