低pH对番茄幼苗菌根丛枝形成和磷营养功能的影响

doi:10.3969/j.issn.1009-7791.2016.04.001

›› 2016, Vol. 45 ›› Issue (04): 301-307.DOI: 10.3969/j.issn.1009-7791.2016.04.001

• 植物生理生化与分子生物学 • 下一篇

低pH对番茄幼苗菌根丛枝形成和磷营养功能的影响

冯曾威,王宁,朱红惠,姚青

(1.华南农业大学园艺学院，广东省微生物信号与作物病害防控重点实验室，广东广州 510642；2.广东省微生物研究所，广东广州 510070)

收稿日期:2016-10-09 修回日期:2017-01-19 出版日期:2016-12-10 发布日期:2016-12-10
通讯作者: 姚青
作者简介:冯曾威，硕士研究生，从事园艺植物生理与生态研究。
基金资助:
国家自然科学基金项目(31570395)

Influences of Low pH on the Arbuscule Formation and Phosphorus Nutrition of Tomato Seedlings

FENG Zeng-wei,WANG Ning,ZHU Hong-hui,YAO Qing

(1.College of Horticulture, South China Agricultural University, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Guangzhou 510642, Guangdong China；2.Guangdong Institute of Microbiology, Guangzhou 510070, Guangdong China)

Received:2016-10-09 Revised:2017-01-19 Online:2016-12-10 Published:2016-12-10
Contact: YAO Qing

摘要/Abstract

摘要： 以番茄Solanum lycopersicum为宿主，在pH 4.5和pH 6.5条件下分别接种3种丛枝菌根真菌(AMF)，即根内球囊霉Rhizophagus irregularis、珠状巨孢囊霉Gigaspora margarita和脆无梗囊霉Acaulospora delicata，探讨低pH对AMF在根系中的丛枝形成和功能的影响。结果表明，低pH能够显著抑制AMF对根系的侵染以及丛枝的形成；3个菌种之间存在差异，表现为珠状巨孢囊霉的侵染强度最高，根内球囊霉的丛枝丰度对低pH最敏感；AMF显著增加番茄的生物量，降低其根冠比；番茄在pH 6.5的菌根依赖性低于在pH 4.5的对应值，对根内球囊霉的菌根依赖性最低；低pH对碱性磷酸酶(ALP)活性的影响与根系侵染有相似的模式；AMF显著提高地上部磷含量，增强根系LePT4的表达，但pH的影响并不显著。以上结果表明，低pH对AMF与植物共生关系的建立和维持有一定的抑制作用，低pH胁迫条件下AMF的促生作用更大，不同AMF菌种/菌株提高植株低pH抗性的能力存在差异。

关键词: 低pH, 丛枝菌根真菌, 丛枝形成, 番茄

Abstract: Tomato inoculated with 3 AMF species, Rhizophagus irregularis, Gigaspora margarita and Acaulospora delicata, at different pH values (4.5 or 6.5), the influences of low pH on the arbuscule formation and function of AMF in tomato roots were investigated. The results indicated that low pH significantly inhibited the AMF colonization and the arbuscule formation in roots. Three AMF species varied greatly with G. margarita showing the highest colonization and R. irregularis showing the most sensitive arbuscule abundance to low pH. AMF significantly elevated the plant biomass and decreased the root to shoot ratio. The effects of low pH on alkaline phosphatase (ALP) activity shared the similar pattern with those on root colonization. AMF significantly increased the shoot phosphorus content and the LePT4 expression in roots; however, pH did not affect them. These results suggested that low pH was inhibitory to the establishment and maintenance of AMF-host symbiosis, and the beneficial effects of AMF was greater at low pH. Moreover, different AMF species/isolates could vary greatly in the ability to increase the resistance of hosts to low pH.

Key words: low pH, arbuscular mycorrhizal fungi, arbuscule formation, tomato

中图分类号:

Q945.12

冯曾威,王宁,朱红惠,姚青. 低pH对番茄幼苗菌根丛枝形成和磷营养功能的影响[J]. , 2016, 45(04): 301-307.

FENG Zeng-wei,WANG Ning,ZHU Hong-hui,YAO Qing. Influences of Low pH on the Arbuscule Formation and Phosphorus Nutrition of Tomato Seedlings[J]. , 2016, 45(04): 301-307.

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低pH对番茄幼苗菌根丛枝形成和磷营养功能的影响

Influences of Low pH on the Arbuscule Formation and Phosphorus Nutrition of Tomato Seedlings

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