关键词: 铁皮石斛, 原球茎, 6-BA, 2,4-D, 增殖, 分化, 离体保存

Abstract: The influence of 6-BA and 2,4-D on proliferation, differentiation and conservation in vitro of Dendrobium candidum was discussed in this paper. The results showed that the better medium for D. candidum protocorm shoot regeneration was MS. The better medium for D. candidum protocorm regenerated bud growth was MS + 6-BA 1mg/L + 2,4-D 0.1 mg/L. The better medium for D. candidum protocorm regenerated bud rooting and protocorm proliferation were also MS. Adding 0.5 mg/L 2,4-D was bad for conservation of D. candidum protocorm, but without 2,4-D or adding 0.1 mg/L 2,4-D contributed to conservation of D. candidum protocorm which had sustained growth trend.

Key words: Dendrobium candidum, protocorm, 6-BA, 2,4-D, proliferation, differentiation, conservation in vitro