关键词: ‘石牌’广藿香, 原生质体分离, 叶肉原生质体

Abstract: Mesophyll protoplasts were isolated and purified from leaves of in vitro propagated Polgostemon cablin (Blanco) Benth. cv. shipaiensis, and different factors affecting protoplasts yield and activity were investigated. The results showed that young leaves of seedling subcultured for 12～22 days in the enzyme solution consisting of 0.5% (w/v) pectolyase Y-23, 0.2%(w/v) Macerozyme R-10 and 1. 5% (w/v) cellulase R-10, in 11% (w/v) mannitol buffered with 0.1% MES and 0.02% CaCl2 for 8 h, the yield was 1.85×107 protoplasts per gram leaves (fresh weight), the viability was above 89%.

Key words: Pogostem cablin cv. shipaiensis, protoplast isolation, mesophyll protoplast