亚热带植物科学 ›› 2013, Vol. 42 ›› Issue (02): 117-120.DOI: 10.3969/j.issn.1009-7791.2013.02.006

• 研究报告 • 上一篇    下一篇

太子参‘柘参1号’离体快繁试验

洪晓云,苏明华,郑诚乐,何恩铭   

  1. 1.福建农林大学 园艺学院,福建 福州 350002;2.福建省亚热带植物研究所,福建省植物生理生化重点公共实验室,福建 厦门 361006
  • 收稿日期:2012-12-03 修回日期:2012-12-03 出版日期:2013-06-10 发布日期:2013-06-10
  • 通讯作者: 苏明华
  • 作者简介:洪晓云(1988-),女,福建泉州人,硕士研究生,从事资源植物生理生化研究。
  • 基金资助:

    福建省公益类科研院所专项(2011R1101022-3)

Rapid Propagation Technique in vitro of Pseudostellaria heterophylla ‘ZheShen No. 1’

HONG Xiao-yun,SU Ming-hua,ZHENG Cheng-le,HE En-ming   

  1. 1.College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian China; 2.Fujian Key Laboratory of Physiology and Biochemistry for Subtropical Plant, Fujian Institute of Subtropical Botany, Xiamen 361006, Fujian China
  • Received:2012-12-03 Revised:2012-12-03 Online:2013-06-10 Published:2013-06-10
  • Contact: SU Ming-hua

摘要: 以太子参‘柘参1号’叶片、茎段为外植体进行离体快繁试验,结果表明:带腋芽的茎段能够诱导出丛生芽,且诱导率较高,最佳诱导培养基为MS + 6-BA 1.0 mg/L + NAA 0.2 mg/L +蔗糖30 g/L;增殖培养基为MS + 6-BA 1.0 mg/L + NAA 0.2 mg/L +蔗糖30 g/L;生根培养基为MS + NAA 0.2 mg/L +蔗糖30 g/L。

关键词: 太子参, 叶片, 茎段, 离体, 快繁

Abstract: Leaves and stem segments of Pseudostellaria heterophylla ‘ZheShen No. 1’ were used as explants to study the rapid propagation technique in vitro. The results showed that stems with axillary buds were better for multiple shoot induction. The medium of multiple shoot induction was MS + 6-BA 1.0 mg/L + NAA 0.2 mg/L + sucrose 30 g/L. The proliferation medium was MS + 6-BA 1.0 mg/L + NAA 0.2 mg/L + sucrose 30-40 g/L. The rooting medium was MS + NAA 0.1-0.2 mg/L + sucrose 30 g/L.

Key words: Pseudostellaria heterophylla, leaf, stem segment, in vitro, rapid propagation

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