A rapid and efficient mini-isolation of DNA for PCR analysis

Subtropical Plant Science ›› 2004, Vol. 33 ›› Issue (01): 29-30.

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A rapid and efficient mini-isolation of DNA for PCR analysis

ZHANG Zhi-zhong¹, WU Jing-hua², LU Liu-xin¹, CHEN Gui-xin¹

1. College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian China;
2. College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian China

Received:2003-09-27 Online:2004-03-10 Published:2004-03-10

一种适于PCR检测的DNA微量提取方法

张志忠¹,吴菁华²,吕柳新¹,陈桂信¹

1. 福建农林大学园艺学院, 福建福州 350002;
2. 福建农林大学生命科学学院, 福建福州 350002

作者简介:张志忠(1976-),男,硕士,从事园艺植物遗传育种与生物技术研究。
基金资助:
福建省"211重点学科"项目经费资助

Abstract

Abstract: A modified method for DNA minipreparation suitable for screening transgenic plants is presented in this paper. The method is rapid and efficient. The total DNA extracted by this method can be used for PCR, RAPD, restriction enzyme digestion.

Key words: DNA, mini-isolation, transgene

摘要： 报道一种简单快速、适于转基因再生植株鉴定的DNA微量提取方法。此法特点:无须研磨,不用液氮,取样量少,操作简便,可在一个离心管中完成;较短时间内可检测大量再生植株,所提取的DNA样品可满足PCR检测要求,结果稳定。

关键词: DNA, 微量提取, 转基因

CLC Number:

ZHANG Zhi-zhong, WU Jing-hua, LU Liu-xin, CHEN Gui-xin. A rapid and efficient mini-isolation of DNA for PCR analysis[J]. Subtropical Plant Science, 2004, 33(01): 29-30.

张志忠,吴菁华,吕柳新,陈桂信. 一种适于PCR检测的DNA微量提取方法[J]. 亚热带植物科学, 2004, 33(01): 29-30.

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A rapid and efficient mini-isolation of DNA for PCR analysis

一种适于PCR检测的DNA微量提取方法

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