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Current Status of Molecular Markers and Genomics of Anoectochilus Herba LIN Shu-qing, MA Xiang-li, XU Wen, HUANG Ze-hao Subtropical Plant Science    2024, 53 (5): 478-486.   DOI: 10.3969/j.issn.1009-7791.2024.05.013 Abstract （109）      PDF（pc） （442KB）（35）       Knowledge map    Save With the development of molecular biotechnology, significant progress has been made in the molecular pharmacognosy research of Anoectochilus herba. This paper summarized the molecular pharmacognostic research on molecular identification, transcriptome sequencing, functional gene cloning and synthesis of Anoectochilus herba, and discussed the problems and prospects of molecular marker biotechnology in the genetic analysis, molecular identification and functional gene discovery, in order to further promote the protection, development and utilization of Anoectochilus herba resources. Reference | Related Articles | Metrics | Comments（0）

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Inoculation with AMF Promotes the Photosynthetic and Antioxidant Capacities of Ponkan Leaves under High Temperature and Drought Stress ZHOU Hong-hui, GE Cong, HUANG Xiao-bing, ZHANG Long, LU Cong-hui, ZHANG Yu-ping, WANG Ping Subtropical Plant Science    2024, 53 (5): 389-398.   DOI: 10.3969/j.issn.1009-7791.2024.05.001 Abstract （98）      PDF（pc） （1709KB）（34）       Knowledge map    Save The effects of inoculation with Arbuscular mycorrhizal fungi (AMF) on the photosynthesis and antioxidant capacity in the leaves of Citrus reticulata ‘Ponkan’ under high temperature and drought stress were investigated. This study would provide Ponkan a production measure for resisting high temperature and drought stress. The experimental treatments were designed as follows: (1) Ponkan without AMF inoculation under high temperature and drought stress; (2) Ponkan with AMF inoculation under high temperature and drought stress; (3) Ponkan without AMF treatment under normal environmental conditions (control). In this study, AMF infection rate in the root and phenotype of Ponkan were observed, and the photosynthetic parameters, reactive oxygen species (ROS) content and antioxidant enzyme activities were measured in the leaves. The results showed that the leaves of Ponkan without AMF inoculation were obviously yellowed and wilted under high temperature and drought stress compared with the control. The net photosynthetic rate, stomatal conductance, transpiration rate, water use efficiency, maximum photochemical efficiency, actual photosynthetic efficiency and photosynthetic electron transport efficiency in the leaves of Ponkan without AMF inoculation were significantly decreased under high temperature and drought stress, while the intercellular CO2 concentration was increased compared with those of control. However, the net photosynthetic rate and stomatal conductance were increased, the intercellular CO2 concentration was decreased, and the photosynthetic capacity was enhanced in the leaves of Ponkan with AMF inoculation compared with those of Ponkan without AMF inoculation under high temperature and drought stress. In addition, the contents of O2܋, H2O2 and malondialdehyde (MDA) in leaves of Ponkan without AMF inoculation were increased while the activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) and ascorbate peroxidase (APX) were decreased under high temperature and drought stress compared with those of control. However, the contents of O2܋, H2O2 and MDA were decreased and the activities of the antioxidant enzymes were increased in the leaves of Ponkan with AMF inoculation compared with those of Ponkan without AMF inoculation under high temperature and drought stress. The results demonstrated that Ponkan growth was inhibited, and the photosynthetic and antioxidant capacities was reduced in the the leaves of Ponkan without AMF inoculation, while the capacities were effectively improved in the leaves of Ponkan with AMF inoculation under high temperature and drought stress. Therefore, we conclude that the higher photosynthetic and ROS scavenging capacities in the leaves of Ponkan with AMF inoculation alleviate the physiological damage of the plants under high temperature and drought stress. Reference | Related Articles | Metrics | Comments（0）

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Identification and Expression Analysis of the TCP Gene Family in Liquidambar formosana ZHAO Jin-tao, YE Xing-zhuang, WANG An-bang, CHEN Zhi-yun, WENG Hui-ying, ZHANG Guo-fang Subtropical Plant Science    2025, 54 (2): 109-120.   DOI: 10.3969/j.issn.1009-7791.2025.02.001 Abstract （82）      PDF（pc） （3585KB）（139）       Knowledge map    Save Liquidambar formosana, a representative deciduous species of the Altingiaceae, holds significant economic and ecological values. The TCP gene family plays a crucial role in plant growth, development, and adaptation to stress. In this study, we integrated the genomic and transcriptomic data of L. formosana and used bioinformatics approaches to identify the LfTCP gene family at the whole-genome level. We analyzed the physicochemical properties of the proteins, gene locations, phylogenetic and evolutionary relationships, intra- and interspecific collinearity, promoter cis-acting elements, and tissue-specific expression profiles. The LfTCP gene family consists of 22 members with protein lengths ranging from 197 to 552 amino acids. These 22 LfTCP genes are unevenly distributed across 12 chromosomes. Phylogenetic analysis indicates that there are 12 members in Class I and 10 members in Class II, including 7 members in the CIN subfamily and 3 members in the CYC/TB1 subfamily. The promoter cis-regulatory elements suggest that the LfTCP gene family may be involved in responses to light, hormones, and stress. Tissue-specific expression analysis shows that LfTCP genes exhibit distinct tissue expression patterns, with LfTCP6, LfTCP7, and LfTCP17 being specifically highly expressed in leaves. These genes can serve as important candidates for studying the growth and morphological development of L. formosana leaves. The results provide a reference for understanding the functions of the LfTCP gene family and the molecular mechanisms by which TCP regulates leaf growth and development in L. formosana. Reference | Related Articles | Metrics | Comments（0）

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Diversity of Floral Traits and Ecological Strategies in 20 Apocynaceae Plants SUN Rui, SONG Peng-fei, YANG Shuai, HAN Mei, YANG Jie Subtropical Plant Science    2024, 53 (5): 433-443.   DOI: 10.3969/j.issn.1009-7791.2024.05.006 Abstract （80）      PDF（pc） （1509KB）（23）       Knowledge map    Save Floral traits, as one of the most diverse and complex collections of traits in life history stages of plants, perform ecological functions such as attraction, defence and resistance to achieve successful reproduction. Flower colour has lagged behind other traits in the study of floral traits, but flower color and its relationship with other floral traits play an important role in attracting pollinators and reproducing offspring in plants. In this study, we analyzed the diversity and correlation of five types of floral traits, including flower color, in 20 species of Apocynaceae, aiming to gain a deep understanding of the diversity of floral traits and their ecological strategies. The results showed that there was a wide diversity of floral traits in Apocynaceae, with significant differences between some floral traits of plants with different flower colors. In addition, we found different degrees of correlation between different floral traits in Apocynaceae, suggesting that plants are able to adopt appropriate ecological strategies through their own needs. Reference | Related Articles | Metrics | Comments（0）

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Estimation of Genome Sizes of Pistacia chinensis by Flow Cytometry and Genome Survey ZHOU Mei-jun, YIN Yue, ZHANG Yong-hong Subtropical Plant Science    2024, 53 (6): 495-502.   DOI: 10.3969/j.issn.1009-7791.2024.06.001 Abstract （80）      PDF（pc） （4084KB）（306）       Knowledge map    Save Pistacia chinensis, a deciduous tree of the genus Pistacia (Anacardiaceae), is an important oil plant resource for its seeds being rich in oil. It has value in wood production and landscape too. Until now, no relevant information on the whole genome of P. chinensis has been found, which limits its resource utilization and germplasm innovation. To determine the whole genome sequencing strategy of P. chinensis, flow cytometry combined with high-throughput sequencing technology was employed to estimate the genome size of this species. The results were as follows: (1) The genome sizes of P. chinensis were estimated to be approximately 480 Mb and 406 Mb by flow cytometry using Solanum lycopersicum and Arabidopsis thaliana as internal references, respectively. (2) 86 Gb of raw data were produced in next-generation sequencing and approximately 85 Gb of clean data were obtained after filtering. (3) NT database comparison found no exogenous pollution in the sequencing data, with five most math species belonging to Anacardiaceae. Pistacia vera, a congeneric species, had the highest sequence similarity with P. chinensis. (4) K-mer analysis showed that the genome size of P. chinensis was 418 Mb with a heterozygosity level of 1.89% and repetitive sequences ratio of 57.5%. The results revealed that the genome of P. chinensis was a complex genome with high heterozygosity and repetitive rates. It is recommended to combine PacBio sequencing technology and Hi-C sequencing in genome assembling with specific concern on heterozygous regions. This study provides valuable reference and important database for the genomics of P. chinensis. Reference | Related Articles | Metrics | Comments（0）

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New Records and New Form of Spermatophyte Distributed in Anhui Province SUN Xiang-yang, SHEN Lang, ZHU Hao, MA Hai-jun, NI Wei-yong, FANG Hong-ming, LI Xiao-hong, SHAO Jian-wen, LIU Kun Subtropical Plant Science    2024, 53 (5): 474-477.   DOI: 10.3969/j.issn.1009-7791.2024.05.012 Abstract （74）      PDF（pc） （1749KB）（27）       Knowledge map    Save Based on field survey and specimen examination, three species and one form are reported as new records in Anhui Province, viz. Elsholtzia argyi Lévl f. albiflora X. Y. Sun & Kun Liu , Euonymus dielsianus Loes. ex Diels, Goodyera velutina Maxim, Ardisia alyxiifolia Tsiang ex C. Chen, in which, Elsholtzia argyi f. albiflora is a new form. This paper briefly describes the distribution, habitat, major identifying features, and differences from similar species for each species with color photos. Reference | Related Articles | Metrics | Comments（0）

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Morphological Supplement to Parahellenia malipoensis (Costaceae) YAN Jia-wei, CHEN Juan Subtropical Plant Science    2024, 53 (5): 468-470.   DOI: 10.3969/j.issn.1009-7791.2024.05.010 Abstract （73）      PDF（pc） （1104KB）（21）       Knowledge map    Save The article offers additional floral morphology of a Costaceae species recently described from Yunnan, namely Parahellenia malipoensis Juan Chen, L.Y. Zeng, S. Jin Zeng & N. H. Xia. This species is readily distinguished from other Parahellenia species by distinctive morphological characters, such as very small stature, unbranched stems, small and plicate leaf blades, small inflorescences and bract with a soft spine at apex, small flowers, and notably short yellow anther crest ornamented with dark red lines. Reference | Related Articles | Metrics | Comments（0）

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Effects of Drought Stress on Root Morphological in Seedlings of Five Species of Garden Plants CHEN Xiao-xi, ZHANG Yan-jing, LAI Can, WU Jia, TAN Can-can, GUO Wei, ZHAN Guo-qiang, SUN Yan-jun Subtropical Plant Science    2024, 53 (6): 520-526.   DOI: 10.3969/j.issn.1009-7791.2024.06.004 Abstract （65）      PDF（pc） （2269KB）（55）       Knowledge map    Save This study investigated the effects of drought stress on the root morphological of urban trees. Two-years-old seedlings of Delonix regia, Spathodea campanulata, Camphora officinarum, Terminalia neotaliala and Ceiba speciosa were used as research subjects. And the changes in root morphology were compared across the different levels of drought stress. The results showed that with the increase of drought stress intensity, the total root length and average connection length of C. officinarum under CK treatment were significantly reduced compared to M and S treatment, and the branch density was significantly increased (P<0.05); The average connection length of T. neotaliala significantly decreased, and the branch density significantly increased. Under drought stress, most root architecture indicators showed a highly significant correlation (P<0.01). As drought stress increased, the correlations among root traits also intensified, particularly under severe drought conditions, where a strong correlation was observed between total root length and the number of root forks. This indicated that under drought stress, tree resource allocation patterns may change significantly, affecting the interrelationships among various root traits. Using principal component analysis (PCA) and maximum variance rotation, the primary structural indicators identified were the number of root forks, total root length, and root crossings. The comprehensive scores of the species in response to drought stress were ranked in descending order as follows: Terminalia neotaliala＞Delonix regia＞Camphora officinarum＞Spathodea campanulata＞Ceiba speciosa. Reference | Related Articles | Metrics | Comments（0）

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Effects of Red and Blue Light Supplementation on Photosynthesis of Tetrastigma hemsleyanum and Its Mechanism HONG Yu-yan, YANG Nan, PAN Meng-ting, LIN Mian-fa, XU Wen, XU Hui-long , FAN Shi-ming Subtropical Plant Science    2024, 53 (5): 408-415.   DOI: 10.3969/j.issn.1009-7791.2024.05.003 Abstract （63）      PDF（pc） （1147KB）（19）       Knowledge map    Save In this study, the effects of red and blue light supplementation on photosynthesis and its regulatory mechanism of Tetrastigma hemsleyanum were investigated, providing a reference for selecting suitable light conditions for T. hemsleyanum. Taking the seedlings of T. hemsleyanum as experiment materials and the white light as the control, the changes of chlorophyll content and net photosynthetic rate of leaves in red and blue light supplementation were measured, and transcriptome sequencing and bioinformatics analysis were performed by Illumina high-throughput sequencing technology. The results showed that the chlorophyll contents and net photosynthetic rates of T. hemsleyanum leaves in red and blue light supplementation were higher than those in white light, and the blue light was significantly higher than those of other treatment groups. A total of 73 598 unigenes were obtained through assembly, among which 1831 differentially expressed unigenes (DEGs) were annotated. A total of 30 DEGs, including 20 enzymes, were identified in the photosynthesis-related pathways of T. hemsleyanum. Red and blue light supplementation could increase the chlorophyll content and net photosynthetic rate of T. hemsleyanum, and the blue light effect had the best effect, which preliminarily revealed the molecular mechanism of red and blue light regulating the photosynthesis of T. hemsleyanum, and laid a foundation for improving the wild domestication and artificial cultivation of T. hemsleyanum. Reference | Related Articles | Metrics | Comments（0）

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Morphological Characteristics and Progress of Floral Bud Differentiation in Cerasus campanulata ZHU Xuan-yi, ZHANG Yang-ting, LI Wan-ying, LIN Wei-jie, ZHAO Kai, Zhou Yu-zhen Subtropical Plant Science    2024, 53 (6): 554-559.   DOI: 10.3969/j.issn.1009-7791.2024.06.008 Abstract （61）      PDF（pc） （9974KB）（185）       Knowledge map    Save To investigate the floral bud differentiation process in different petal types of Cerasus campanulata, flower developments of three varieties were observed by paraffin sectioning method in this study, i.e. the single-petaled type Cerasus ×kanzakura ‘Praecox’, double-petaled type C. campanulata ‘Double-flowered’ and semi-double-petaled type C. kanzakura×campanulata ‘Satis in Pink’. The results demonstrated that all three varieties underwent six distinct stages of floral bud differentiation: initial differentiation period, inflorescence primordium differentiation period, sepal differentiation period, petal differentiation period, stamen differentiation period, and pistil differentiation period. During the petal and stamen differentiation stages, the three varieties showed significant regularity. The petal differentiation period progressively lengthened in the varieties ‘Praecox’, ‘Satis in Pink’, and ‘Double-flowered’, while the stamen differentiation period gradually shortened. It is believed that the formation of double flower of C. campanulata varieties mainly comes from stamen petal, which belongs to the origin of pistil and stamen. The semi-double-petaled ‘Satis in Pink’ had a floral bud differentiation cycle of 98 days, the single-petaled ‘Praecox’ took 105 days, and the double-petaled ‘Double-flowered’ required 112 days. The differentiation process in various floral forms of C. campanulata was largely similar, with stamen petalization contributing to the development of double flowers. This study provides a morphological basis for further investigation into the causes and molecular mechanisms underlying double flower formation in C. campanulata. Reference | Related Articles | Metrics | Comments（0）

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Effects of Exogenous Gibberellin on Rice Seed Germination and Seedling Growth under Salt Stress QIAO Jiao Subtropical Plant Science    2024, 53 (6): 512-519.   DOI: 10.3969/j.issn.1009-7791.2024.06.003 Abstract （60）      PDF（pc） （475KB）（126）       Knowledge map    Save This experiment investigated the effects of different concentrations of gibberellin (25, 50, 100 mg·L–1) on rice seed germination and seedling growth under sodium chloride stress. The results showed that during the seed germination stage, exogenous gibberellin enhances the germination of rice seeds under salt stress, with an increase in germination vigor, germination rate, and germination index, and a decrease in relative damage rate. During the growth stage of seedlings, morphological analysis showed that exogenous gibberellin alleviated the inhibition of salt stress on seedling growth, resulting in an increase in plant height, root length, root tip number, and leaf area; Physiological and biochemical analysis showed that exogenous gibberellin improved the activity of superoxide dismutase (SOD) in rice leaves under salt stress, reduced the content of malondialdehyde (MDA) in leaves, increased the content of photosynthetic pigments including chlorophyll a, b and carotenoids, enhanced photosynthesis, increased the effective quantum yield of PSⅡ [Y(Ⅱ)] and the photochemical quenching coefficient (qP), and decreased the non photochemical quenching coefficient (NPQ). In summary, exogenous gibberellin soaking treatment significantly promoted the germination of rice seeds under salt stress, the effects of sodium chloride stress on seedling growth and development was significantly reduced after spraying exogenous gibberellin, and the optimal concentration of gibberellin was 50 mg·L–1. The research results provide a theoretical basis for optimizing rice cultivation in saline soil. Reference | Related Articles | Metrics | Comments（0）

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Cheirostylis sherriffii, a Newly Recorded Species of Orchidaceae from China FU Xiao-ying, XIONG Chi Subtropical Plant Science    2024, 53 (5): 471-473.   DOI: 10.3969/j.issn.1009-7791.2024.05.011 Abstract （60）      PDF（pc） （1076KB）（30）       Knowledge map    Save Cheirostylis sherriffii N. Pearce & P. J. Cribb was reported as a newly recorded species of Orchidaceae from China. The report included characteristic descriptions and color photos. This species was similar to C. glandulifera (Aver.) J. M. H. Shaw, but could be distinguished by its erect stems, short rhizome, 3-6 flowered inflorescence, and pale green sepals and petals. Voucher specimens were deposited in the Herbarium of Guangxi Institute of Botany (IBK). Reference | Related Articles | Metrics | Comments（0）

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Effects of Salicylic Acid Treatment on Physiology Quality of Postharvest Longan Fruit WANG Hui-ling, SUN Jun-zheng, LIU Qing-qing, MING Yan-lin, LIN He-tong, CHEN Yi-hui Subtropical Plant Science    2024, 53 (6): 527-536.   DOI: 10.3969/j.issn.1009-7791.2024.06.005 Abstract （57）      PDF（pc） （802KB）（34）       Knowledge map    Save Longan (Dimocarpus longan) fruit has high nutritional value. However, the fruit matures during the season of high temperature and high humidity, from July to September. The postharvest physiological metabolism of longan fruit is vigorous. After 3 or 4 days of storage at room temperature, the endocarp exhibited browning, and the pulp was breakdown, which finally caused deterioration. In this study, ‘Fuyan’ longan fruit was used as material to evaluate the effects of salicylic acid (SA) treatment on postharvest physiological quality of Phomopsis longanae-inoculated longan fruit stored at 28 ℃. The results showed that SA treatment decreased the indices of pericarp browning, pulp breakdown, and fruit weight loss percentage. On the 5th day of storage, the pericarp browning index, pulp breakdown index, and fruit weight loss rate in the treatment group of SA+P. longanae were 7.65%, 15.91%, and 24.57% lower than those of the control group of P. longanae. Meanwhile, SA treatment delayed the decrease in L* value, and contents of chlorophyll, carotenoid, anthocyanin, flavonoids, and total phenolic in the pericarp, maintained higher contents of total sugar, sucrose, total soluble solids (TSS) and vitamin C in the pulp of longan fruit, and delayed the increase of titratable acids (TA) and reducing sugar contents in longan fruit. This study proved that SA treatment could maintain better physiological quality and improve the storability of harvested longan fruit. Reference | Related Articles | Metrics | Comments（0）

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A New Hedychium Cultivar ‘Fensi’ WANG Jin-ying Subtropical Plant Science    2024, 53 (6): 592-594.   DOI: 10.3969/j.issn.1009-7791.2024.06.013 Abstract （57）      PDF（pc） （2324KB）（15）       Knowledge map    Save Hedychium ‘Fensi’ is a new cultivar developed through artificial cross-pollination between the wild species white-flowered Hedychium coronarium (as the female parent) and the cultivar Hedychium ‘Fireflies’ (as the male parent). It was selected from individual plants of the F1 generation and propagated through division. This cultivar has high ornamental value. The flowers are elegant in color, with pale yellow labellums and pink centers. The filaments are slender and pink, creating a warm yet refreshing color scheme that remains unchanged until the flowers wilt. Another notable feature is the filamentous wrinkles on the labellums, which are distinctly different from those of its parents, hence the name ‘Fensi’. The average height of a two-year-old mature plant is 105.2 cm, which is between the height of the female parent (258.3 cm) and the male parent (100.1 cm). The fragrance of the flowers is slightly less intense than that of the female parent, while the male parent has no fragrance. Reference | Related Articles | Metrics | Comments（0）

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Comprehensive Quantitative Evaluation of Cutting Rooting Ability of Rosa chinensis Varieties CHEN Juan, ZHENG Tian-rui, LI Shi-feng, LI Wei, ZHANG Hao, SONG Jie Subtropical Plant Science    2024, 53 (5): 459-467.   DOI: 10.3969/j.issn.1009-7791.2024.05.009 Abstract （55）      PDF（pc） （1406KB）（16）       Knowledge map    Save To comprehensively evaluate the rooting ability of different types and varieties of rose, and select the varieties with a strong rooting ability to provide reference for the future production and breeding of rose varieties, 59 rose varieties introduced and cultivated in Kunming, Yunnan Province, China were used as experimental materials, the root morphological indexes of rose cuttings were measured by WinRHIZO root analysis system, and the rooting ability of each rose variety was comprehensively quantitatively evaluated by principal component analysis and membership function analysis. The distribution of the root indexes of rose cuttings basically conformed to the normal distribution, except for the number of branches, the difference of the root indexes was small. Principal component analysis transformed 7 single indicators into 2 independent factors, with a cumulative contribution rate of 89.53%. Through membership function analysis, it was found that there were 23 varieties with a comprehensive score ≥0.40, accounting for 38.98%. In this study, 10 rose varieties with the strongest rooting ability were selected, including ‘Angela’, ‘Rouge Royal’, ‘Red Apple rose’, ‘Clg White Eden Rose’, ‘Highgrove’, ‘Pink Flamingo’, ‘Fensemenghuan’, ‘New Imagine’, ‘Billet Doux’ and ‘Crown Princess Margareta’, which can be used as a breed for large-scale production and as a parent for genetic improvement. Reference | Related Articles | Metrics | Comments（0）

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Determination of 10 Phenylethanol Glycosides in Cistanche deserticola from Different Origins and 2 Species of Host Plants Based on UPLC-QQQ-MS/MS ZHAO Fa-fa, GUO Ye-hong, GAO Pei-wen, CHEN Jin-hui, ZHANG Wen-yao Subtropical Plant Science    2024, 53 (5): 399-407.   DOI: 10.3969/j.issn.1009-7791.2024.05.002 Abstract （54）      PDF（pc） （947KB）（75）       Knowledge map    Save Ultra performance liquid chromatography-triple quadrupole tandem mass spectrometry (UPLC-QQQ- MS/MS) was used for the simultaneous determination of 10 phenylethanol glycosides in Cistanche deserticola of 2 host species from different origins. The results showed that the linearity of the 10 components to be measured was good in their respective ranges (R2＞0.9989), and the average recoveries of the controls were in the range from 94.61% to 102.71%, with the RSD values less than 3.39%, which indicated that the results of the established method were accurate. The total amount of phenylethanol glycosides of C. deserticola from Inner Mongolia was significantly higher than those of C. deserticola from Xinjiang and Gansu. The total amount of phenylethanol glycosides of Atriplex canescens – C. deserticola was in the order of Inner Mongolia＞Xinjiang＞Gansu, while the total amount of phenylethanol glycosides of Haloxylon ammodendron – C. deserticola was in the order of Inner Mongolia＞Gansu＞Xinjiang. The total amount of phenylethanol glycosides of A. canescens – C. deserticola from the 3 origins was higher than those of H. ammodendron – C. deserticola. It provided a more convenient assay for the determination of phenylethanol glycosides among C. deserticola of different origins and different hosts, and also provided scientific ideas for the quality evaluation and host selection of C. deserticola. Reference | Related Articles | Metrics | Comments（0）

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Introduction of Nomenclature Section of ⅩⅩ International Botanical Congress MA Jin-shuang Subtropical Plant Science    2024, 53 (5): 487-494.   Abstract （54）      PDF（pc） （1082KB）（20）       Knowledge map    Save Reference | Related Articles | Metrics | Comments（0）

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Ultrasound-Assisted Deep Eutectic Solvent Extraction of Gymnema sylvestre Polysaccharides and Its Activity Determination HAO Sheng-jie, HOU Hai-qing, YIN Jie, LI Hong-sen, DU Yu-xin, JIN Feng-yang, LI Qian, CHEN Gui-lin Subtropical Plant Science    2024, 53 (6): 545-553.   DOI: 10.3969/j.issn.1009-7791.2024.06.007 Abstract （51）      PDF（pc） （3283KB）（30）       Knowledge map    Save Optimization of ultrasonic-assisted deep eutectic solvents (DESs) extraction process for Gymnema sylvestre polysaccharides was conducted to investigate its biological activity, to provide theoretical support for the development of G. sylvestre products. Using the polysaccharide extraction yield as the evaluation index, the Box-Behnken response surface method was applied to optimize the extraction process based on single-factor experiments. Additionally, the antioxidant activities of the polysaccharides were evaluated by their ability to scavenge DPPH, ABTS+, FRAP, and H2O2 radicals, while the inhibitory effects on α-amylase and α-glucosidase, as well as antibacterial activity against several bacteria strains, were also studied. The results showed that the DESs synthesized from choline chloride and citric acid achieved the highest extraction rate. After optimization using the response surface method, under conditions where the molar ratio of choline chloride to citric acid was 1:1.02, water content was 39.32%, liquid-to-material ratio was 20.21:1, ultrasonic time was 39.23 min, ultrasonic temperature was 41.35 ℃, and ultrasonic power was 412 W, the extraction yield of G. sylvestre polysaccharides reached 29.23%, which was nearly 15 times higher than the traditional water extraction method. In vitro activity experiments demonstrated that G. sylvestre polysaccharides exhibited high antioxidant activity, broad-spectrum antibacterial capacity, and enzyme inhibition activity, showing great potential for developing novel anti-diabetic products. DESs, being environmentally friendly, efficient, and reusable as extraction media for natural products, significantly improved the extraction efficiency of G. sylvestre polysaccharides. Reference | Related Articles | Metrics | Comments（0）

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Selection of Reference Genes for Quantitative Real-Time PCR in Cymbidium ensifolium GUO Li-ting, CHEN Juan, LI Yao, LI Jin-jin, TIAN Yang, AI Ye Subtropical Plant Science    2024, 53 (5): 425-432.   DOI: 10.3969/j.issn.1009-7791.2024.05.005 Abstract （51）      PDF（pc） （1260KB）（16）       Knowledge map    Save In this study, different organ tissues (floral organs: sepal, petal, lip and gynostemium; vegetative organs: root, pseudobulb, and leaf) of Cymbidium ensifolium ‘Dafengsu’ were used as materials, to screen out the stably expressed reference genes for real-time quantitative PCR (qRT-PCR). The expression of five candidate internal reference genes (Actin, 18SrRNA, rpoB, GAPDH, EF-1a) in different organ tissues of ‘Dafengsu’ was analyzed by qRT-PCR. The expression stability of candidate reference genes was comprehensively evaluated by geNorm, NormFinder and Bestkeeper, and the selected reference genes were verified. The results indicated that the expression stability of candidate reference genes was varied, and GAPDH and Actin were the best stable reference genes in floral and vegetative organs in different parts of ‘Dafengsu’, respectively. This study can provide reference for the selection of reference genes in the gene expression research of C. ensifolium. Reference | Related Articles | Metrics | Comments（0）

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Effects of Pinus massoniana Invasion on Tree Growth and Soil of Chinese fir and Its Cyclobalanopsis elevaticostata Mixed Forest CHEN Ming-jiu Subtropical Plant Science    2024, 53 (5): 444-450.   DOI: 10.3969/j.issn.1009-7791.2024.05.007 Abstract （51）      PDF（pc） （1014KB）（16）       Knowledge map    Save This study investigated the competitive growth dynamics between Pinus massoniana and Chinese fir (Cunninghamia lanceolata), and examined the influence of their respective mixed forest compositions on tree growth and soil physicochemical properties. The research focused on Chinese fir stands naturally invaded by P. massoniana through seed wind dispersal, as well as mixed forests P. massoniana and Chinese fir in combination with broadleaf species Cyclobalanopsis elevaticostata. Tree growth indices and soil physicochemical characteristics were measured and analyzed across these forest types. The main findings were as follows: (1) In the early stages, mixed broadleaf-conifer forests exhibited higher canopy closure and superior tree height, diameter at breast height (DBH), and volume growth compared to pure P. massoniana and Chinese fir mixed forests, despite a lower stand density due to higher mortality rates of broadleaf trees. Furthermore, P. massoniana demonstrated better growth than Chinese fir in both forest types. (2) Soil physical properties showed no significant variation across different soil layers within the same forest type. However, soil bulk density increased with depth, while capillary water-holding capacity and capillary porosity decreased in mixed broadleaf-conifer stands. (3) Soil pH was consistently below 4.5 and no difference in the same stand. The concentrations of total potassium, total phosphorus, organic matter, and alkali-hydrolyzable nitrogen were higher in mixed broadleaf-conifer forests compared to P. massoniana and Chinese fir mixed forests, although the levels of available potassium and phosphorus were lower. In conclusion, natural seed invasion of P. massoniana proved strong, with this species demonstrating greater suitability for growth in the region. Mixed broadleaf-conifer forests were more effective in enhancing soil fertility compared to pure P. massoniana and Chinese fir stands, with P. massoniana exhibiting superior growth in both forest types. These findings provided valuable insights for the sustainable management of artificial forests. Reference | Related Articles | Metrics | Comments（0）

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Comprehensive Evaluation on the Ornamental Value of 13 Paeonia lactiflora Varieties Based on Analytic Hierarchy Process YING Meng-yun, SHI Jian-yu, YE Qiu-ping, SHEN Qing-yu, YU Wen Subtropical Plant Science    2024, 53 (6): 577-583.   DOI: 10.3969/j.issn.1009-7791.2024.06.011 Abstract （51）      PDF（pc） （496KB）（22）       Knowledge map    Save Thirteen high-quality ornamental varieties of Paeonia lactiflora were collected and observed in this study. Selecting indicators based on a comprehensive evaluation model for the ornamental value of varieties. Thirteen ornamental indicators based on flower traits, plant traits, and growth properties were selected to comprehensively evaluate the ornamental values of P. lactiflora varieties using analytic hierarchy process (AHP) method. The results showed that according to the constructed judgment matrix, the weight of flower color, plant type, and growth potential in the indicator layer were relatively high, which was particularly important in the evaluation of the ornamental value of P. lactiflora. These varieties were divided into gradeⅠ,Ⅱand Ⅲ based on the comprehensive scores. The four varieties of gradeⅠhad the best ornamental effects, including ‘Xueyuanhongxing’, ‘Fengyuluojinchi’, ‘Qihualushuang’ , ‘Zhongshengfen’ , which had good adaptability and ornamental value. They were advisable to leverage their advantages for promotion. The comprehensive performance of the six varieties of grade Ⅱ was average, including ‘Moziling’, ‘Dafugui’, ‘Qingwen’, ‘Lanfushi’, ‘Luhong’, ‘Yanzixiangyang’. The comprehensive evaluation value of grade Ⅲ was relatively low, including ‘Hongfushi’, ‘Hongyanzhenghui’, ‘Zifengzhaoyang’. The comprehensive evaluation results of the ornamental value of P. lactiflora based on the analytic hierarchy process could provide reference for the application of P. lactiflora resources in ornamental horticulture. Reference | Related Articles | Metrics | Comments（0）

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Subtropical Plant Science    2024, 53 (6): 595-.   Abstract （48）      PDF（pc） （207KB）（18）       Knowledge map    Save Related Articles | Metrics | Comments（0）

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Root Structure Characteristics of Phyllostachys edulis Forests and P. praecox Forests LÜ Hui-fei, WANG Ling-ling, ZHAO Zi-qing, SUN Han-jing, ZHENG Hui Subtropical Plant Science    2024, 53 (5): 451-458.   DOI: 10.3969/j.issn.1009-7791.2024.05.008 Abstract （48）      PDF（pc） （548KB）（23）       Knowledge map    Save The study of differences in root structure characteristics between Phyllostachys edulis and P. praecox forests through measurements of length, volume, surface area, and dry weight of the root was carried out, which provided a basis for bamboo resource cultivation, soil management and enhancing the economic benefits of bamboo forests. The soil distribution characteristics of bamboo forest roots were investigated by using the methods of root digging scanning. P. edulis roots mainly distributed in the soil layer of 0-40 cm, and the root surface area, volume, length and dry mass accounted for more than 80% of the total root system. The proportions of root length and volume of different diameter classes in P. edulis and P. praecox forests showed the decreasing trend with the deepening of the soil layer. In the different diameter classes of P. edulis roots, roots with diameters＜1 mm had the highest proportion of length (70.45%), whereas those with diameters＞2 mm had the highest proportion of volume (78.73%). In P. praecox forest, the roots were distributed only in the soil layer of 0-40 cm, and the root volume, surface area, and dry weight accounted for more than 60% in the surface soil layer (0-20 cm). The root length accounted for 59.39% of the total length, and roots with diameters＜1 mm had the highest proportion of length (80.46%) among different diameter classes, though they had the smallest proportion of volume (16.63%). In the soil layer of 0-40 cm, the proportion of root length in three diameter classes showed that P. praecox forest was greater than P. edulis forest. The proportion of roots with diameters≤2 mm showed that P. edulis forest was less than P. praecox forest. The proportion of roots with diameters＞2 mm in P. edulis forest was greater than P. praecox forest. The soil layer of 0-40 cm is the main active layer for the growth of bamboo forest roots, and it is also the important level for soil management in bamboo forests. P. edulis and P. praecox differ significantly in root content and structure. P. edulis has the larger root structure compared to P. praecox. Therefore, it is necessary to propose measures that are more suitable for bamboo management by combining different root structure characteristics. Reference | Related Articles | Metrics | Comments（0）

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Genome Size Estimation of Uraria lagopodioides and Christia vespertilionis (Fabaceae) by Flow Cytometry GAO Ying-jie, MIAO Jia, HUANG Xiang, ZHAO Xue-li Subtropical Plant Science    2024, 53 (6): 503-511.   DOI: 10.3969/j.issn.1009-7791.2024.06.002 Abstract （47）      PDF（pc） （808KB）（22）       Knowledge map    Save This study aimed to determine the genome size of medicinal plants Uraria lagopodioides and Christia vespertilionis (Fabaceae), and to explore the appropriate experimental conditions. We screened five nuclear dissociation buffer and two methods for preparing nuclear suspensions, and then we estimated the genome size of both species by flow cytometry using the most appropriate experimental conditions with Zea mays as an internal standard plant. The results revealed that MGB was a suitable nuclear dissociation buffer for flow cytometry of both plants, and filtering through the 40 μm mesh for three times or centrifugation at 1000 r·min–1 was the appropriate method for preparing nuclear suspensions. The genome size of U. lagopodioides and C. vespertilionis were estimated to be 0.69 Gb and 0.64 Gb, respectively. Genome size estimation and the exploration of suitable experimental conditions for flow cytometry in this study can provide reference for the genomics, genetic evolution, and resource development of U. lagopodioides and C. vespertilionis. Reference | Related Articles | Metrics | Comments（0）

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Analysis of the Current Status and Spatial Distribution of Ancient and Famous Trees in Xiamen City WANG Long-ping Subtropical Plant Science    2024, 53 (6): 569-576.   DOI: 10.3969/j.issn.1009-7791.2024.06.010 Abstract （46）      PDF（pc） （2254KB）（21）       Knowledge map    Save This study analyzed the current status and spatial distribution characteristics of ancient and famous trees in Xiamen City, with the aim of gaining a deeper understanding of the situation of these resources and providing a scientific basis for their protection and management. Based on data from the 2021–2023 survey of ancient and famous trees in Xiamen, the study employed a combination of GIS information technology and traditional analysis methods to examine three diversity indices of ancient and famous trees, as well as their distribution in relation to administrative regions, topography, slope, and aspect. Xiamen City has a total of 1773 ancient and famous trees registered in its records，including 11 famous trees, 68 first-grade ancient trees, 319 second-grade ancient trees, and 1375 third-grade ancient trees, belonging to 43 species of 39 genera in 27 families. The proportion of those with normal growth potential is 82.18%. The species are predominantly native, with banyan trees being the most numerous. The Shannon-Wiener (H) index for biodiversity is 1.588, the Pielou evenness index is 0.420, and the Margalef richness index is 5.882, indicating a moderate level of biodiversity. The nearest neighbor ratio for ancient and famous trees in Xiamen City is 0.30, which is less than 1, indicating a clustered spatial distribution type. The highest density is found in Gulangyu, Siming District, exceeding 26.9 trees per square kilometer. 94.6% of these trees are located on slopes less than 15 degrees, and they are evenly distributed across various aspects. Among different land use types, residential and construction land has the highest proportion of ancient and famous trees, accounting for 63.8%, followed by farmland at 29.9%. Reference | Related Articles | Metrics | Comments（0）

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Full-length Transcriptome Sequencing Analysis of Medicinal Plant Pithecellobium clypearia ZHONG Feng-di, LING Teng-hong, MOU Gui-ping, ZHU Kong-yu, WANG Peng-long, YAN Xiao-dong Subtropical Plant Science    2024, 53 (5): 416-424.   DOI: 10.3969/j.issn.1009-7791.2024.05.004 Abstract （45）      PDF（pc） （1099KB）（22）       Knowledge map    Save As the plant with remarkable medicinal value, the insufficiency of genomic information of Pithecellobium clypearia restricts its in-depth comprehension. In this study, PacBio Sequel sequencing technology was employed to conduct full-length transcriptome sequencing of the mixed samples from roots, mature branches, young branches, mature leaves, and young leaf tissues of P. clypearia. The results showed that 118 472 unique transcripts were successfully identified and further categorized into 44 939 Unigenes. After comparison with the Nr, Uniprot, GO, KEGG, and KOG databases, functional annotations were provided for 37 198 Unigenes, and the majority of these Unigenes were involved in biological processes and metabolic pathways. Simultaneously, 13 897 Unigenes were discovered to undergo alternative splicing, including intron retention, 3' end splicing diversity, and 5' end splicing diversity. Additionally, 377 and 159 structural genes involved in the biosynthesis of flavonoids and terpenoids, as well as 132 and 180 Unigenes encoding members of the MYB and WRKY transcription factor families, were identified. This study expanded the genomic resources of P. clypearia, provided valuable data support for exploring the metabolic regulatory mechanism of its bioactive components, screening of key genes, and development of molecular markers, laid a foundation for further exploitation of the medicinal value of P. clypearia in future. Reference | Related Articles | Metrics | Comments（0）

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Auxin Signaling Pathway-Mediated Regulation of Lateral Root Formation of Maize Plants by Arbuscular Mycorrhizal Fungus ZHU Feng-wa, CHEN Wei-li, ZHANG Wei, CHEN Mei-zheng, LIU Xiao-di, YAO Qing Subtropical Plant Science    2025, 54 (2): 132-143.   DOI: 10.3969/j.issn.1009-7791.2025.02.003 Abstract （45）      PDF（pc） （3238KB）（45）       Knowledge map    Save Arbuscular mycorrhizal (AM) fungi have the capacity to alter plant lateral root (LR) formation, a process that is highly dependent on phosphorus (P) levels and plant hormones. This study using maize (Zea mays) as test plants, Rhizophagus irregularis was inoculated in soils with no P application (soil available P of 20.5 mg·kg–1, namely low P level) and additional P application of 30 mg P·kg–1 as KH2PO4 (namely high P level). Plant biomass, LR number, the contents of indole-3-acetic acid (IAA) and zeatin riboside (ZR) in roots were determined, and the expression of LR formation-related genes (ZmKRP2, ZmPSK1, and ZmPSK3) and auxin signaling-related genes (ZmNAC1, ZmARF1, ZmTIR1, ZmPIN1b, and ZmAUX1) was also quantified. At 35 days after sowing, AM fungal significantly increased maize biomass at both phosphorus levels compared with no inoculation. AM fungal inoculation significantly increased the numbers of 2nd LR and 3rd LR by 53.5%–237.8%, while the effect of P levels on LR number was relatively weak. AM fungal inoculation significantly enhanced the expression of ZmPSK3, and P levels enhanced the expression of ZmPSK1, ZmPSK3, and ZmKRP2. Additionally, IAA contents and signaling pathways were significantly affected by both P levels and AM fungal inoculation. When inoculated with AM fungus, IAA content at 35 days after sowing at low P level was significantly increased by 48.4% than that at high P level. AM fungal inoculation significantly upregulated the expression of auxin signaling-related genes. Compared with 1ow P level, high P level significantly increased the expression of ZmNAC1, but decreased the expression of ZmAUX1. The findings indicated that ZR content was not affected by P level and AM fungus, while the auxin signaling pathway was involved in the LR formation mediated by AM fungal inoculation, whose effect magnitude was more pronounced that that of P levels. Reference | Related Articles | Metrics | Comments（0）

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Screening of Selenium-enriched Dendrobium Endophytes and Its Dynamic Changes of Selenium Enrichment LIU Ru-zhen, XU Wei, LUO Zhu-hua, GUO Chi-ming, WANG Jie, LIN Wen-zhen Subtropical Plant Science    2024, 53 (6): 537-544.   DOI: 10.3969/j.issn.1009-7791.2024.06.006 Abstract （41）      PDF（pc） （6698KB）（15）       Knowledge map    Save This study aimed to identify and characterize strains from Dendrobium officinale with high selenium tolerance and enrichment capacity, and to investigate the dynamics of selenium accumulation in these strains. The findings are intended to provide a theoretical foundation for the improvement of selenium-enriched microbial germplasm and for enhancing selenium content in plants. The strain BK-1 was initially identified through 16S rDNA sequence analysis. The strain’s tolerance to sodium selenite was then evaluated, determining its maximum tolerance concentration. The ability of the strain to convert inorganic selenium into organic selenium during the enrichment process was assessed, along with measurements of total selenium content, organic selenium yield, and conversion efficiency. An optimization experiment was also conducted to establish the optimal selenium ion concentration for supplementation. Strain BK-1 was identified as Burkholderia gladioli. This strain demonstrated a high tolerance to sodium selenite, with a maximum tolerance concentration of 130 μg·mL–1. During the selenium enrichment process, strain BK-1 achieved a total selenium content of 577.74 μg·g–1 DCW (dry cell weight ) within 48 hours, with an organic selenium yield of 505.66 μg·g–1 DCW and a conversion efficiency of 87.42%. The addition of selenium ions significantly shortened the logarithmic growth phase and reduced biomass, with the optimal selenium ion concentration determined to be 50 μg·mL–1. Reference | Related Articles | Metrics | Comments（0）

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Design on Vectorized Expression Scheme of Plant Microscopic Tissue Structure LIU Qing, XING Qian, CHE Su-rong Subtropical Plant Science    2024, 53 (6): 584-591.   DOI: 10.3969/j.issn.1009-7791.2024.06.012 Abstract （39）      PDF（pc） （2273KB）（13）       Knowledge map    Save Based on traditional plant microstructure schematic drawing, this study explored a vectorized expression scheme with the aim of overcoming the shortcomings of ink line drawing, reducing workload, enhancing expressiveness, and improving the efficiency of reading diagrams. Using the computer vector graphics software CorelDRAW, we took photos of plant microstructure cross-sections as the basis and combined them with conventional practices for drawing plant ink line drawing. The outlines of plant structural features were drew without adding hatching lines, crosshatching, or random dots for filling styles. Instead, appropriate color blocks and necessary colored lines were used to represent the structural characteristics of plant microstructures. This method was successfully used to create vector expression diagrams of plant microscopic tissue structure, and six color-matching techniques were summarized. The vector expression diagram of plant microscopic tissue structure drawn using this method is easy to operate and modify. It reduced workload, improved the efficiency of reading diagrams, and had minimal impact on structural features when zooming in or out, making it convenient for editing and printing. Reference | Related Articles | Metrics | Comments（0）

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Genome-wide Identification and Tissue Expression Analysis of the KNOX Gene Family in Altingia chinensis WANG An-bang, YE Xing-zhuang, ZHAO Jin-tao, CHEN Zhi-yun, WENG Hui-ying, LIN Mao, ZHANG Guo-fang Subtropical Plant Science    2025, 54 (1): 1-10.   DOI: 10.3969/j.issn.1009-7791.2025.01.001 Abstract （35）      PDF（pc） （2586KB）（209）       Knowledge map    Save The KNOX gene family has important regulatory functions in leaf-shape growth and development in plants. This paper aims to analyze the KNOX gene family of Altingia chinensis and explore its tissue expression pattern. We used bioinformatics methods to identify and study AcKNOXs at the whole genome level of A. chinensis, and analyzed the gene location, gene structure, phyloevolutionary relationships, gene collinearity and the transcriptome expression. We identified 11 AcKNOXs from 8 chromosomes in the A. chinensis genome. Among them, AcKNOX6 and AcKNOX9 lack ELK and Homeobox KN domains, AcKNOX7 lacks ELK domain, the others have four conserved domains: KNOX 1, KNOX 2, ELK and Homeobox KN. 11 AcKNOXs are classified into Class Ⅰ (AcKNOX1, AcKNOX2, AcKNOX5, AcKNOX8, AcKNOX10, AcKNOX11), Class Ⅱ (AcKNOX3, AcKNOX4, AcKNOX7) and Class M (AcKNOX6, AcKNOX9). The AcKNOXs protein contains 144 to 444 amino acids. They were all located in the nucleus and are all acidic, unstable and hydrophilic. Through the expression pattern analysis, we found that AcKNOXs genes have tissue specificity. The majority of the members of the same subfamily showed the similar expression pattern. The main amplification mode of 11 AcKNOXs were segmental duplication, no tandem repeats were found. There were four pairs of genes with collinearity, AcKNOX3/AcKNOX4, KNOX2/AcKNOX8, AcKNOX6/AcKNOX9, and AcKNOX5/AcKNOX10, respectively. The results of this research provide the study of KNOX gene family with scientific basis, which help to do in-depth study on the genomic characteristics. Reference | Related Articles | Metrics | Comments（0）

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New Records of Seed Plants from Guangdong Province Ⅵ XIONG Qin-dai, XUE Jian-jun, LUO Wu-hong, ZENG Qiu-gen, WANG Shi-yang, LAI Xiao-chao, DENG Jun, KANG Ning, FAN Qiang Subtropical Plant Science    2025, 54 (2): 209-216.   DOI: 10.3969/j.issn.1009-7791.2025.02.011 Abstract （35）      PDF（pc） （2327KB）（56）       Knowledge map    Save Six new records of seed plants in Guangdong Province are reported, namely Pinus fenzeliana Hand.-Mazz., Stemona japonica (Bl.) Miq., Scutellaria yunnanensis H. Lév., Ehretia densiflora F. N. Wei & H. Q. Wen, Elatostema pycnodontum W. T. Wang and Rubus jianensis L. T. Lu &Boufford. These new records enrich the plant diversity in Guangdong Province, which are of great significance for the study of the flora of seed plants in Guangdong Province. Reference | Related Articles | Metrics | Comments（0）

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Evaluation of Cold Resistance of 9 Clones of Acacia mangium × A. auriculiformis LIAO Fang-yan, CHEN Bo-zhen, WANG Li-han, WANG Yu-xin, LI Na, ZHU Bao-zhu, ZHANG Wei-hua Subtropical Plant Science    2025, 54 (1): 45-53.   DOI: 10.3969/j.issn.1009-7791.2025.01.006 Abstract （33）      PDF（pc） （638KB）（54）       Knowledge map    Save The seedlings of 9 clones of Acacia mangium × A. auriculiformis  were used as experimental materials, and the cold environment was simulated by a large low-temperature artificial climate chamber. Physiological indexes such as relative electrical conductivity (REC), soluble protein (SP), free proline (Pro) and malondialdehyde (MDA) were determined, and the response of physiological indexes, cold resistance were analyzed under low temperature conditions, and the cold resistance of 9 clones of A. mangium × A. auriculiformis were evaluated by membership function method comprehensively. Results showed that the relative electrical conductivity and soluble protein content of leaves of different clones increased gradually, the free proline content and SOD activity increased first and then decreased, and the MDA content increased first and then decreased and then increased under low temperature stress. According to the average membership function value, the rank of the cold resistance of 9 clones of A. mangium×A. auriculiformis is No. 8 > No. 7 > No. 6 > No. 2 > No. 1 > No. 9 > No. 3 > No. 4 > No. 5. The No. 8 has the strongest cold resistance, and it can be used as a high quality cold resistance material for hybrid breeding and cultivation. Reference | Related Articles | Metrics | Comments（0）

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Research Progress of TCP Transcription Factors in Orchidaceae CHEN Jia-yi, HUANG Ting-ting, XIONG Long-wei, LI Peng, GUO Li-ting, AI Ye Subtropical Plant Science    2025, 54 (2): 229-238.   DOI: 10.3969/j.issn.1009-7791.2025.02.014 Abstract （33）      PDF（pc） （535KB）（18）       Knowledge map    Save The TCP transcription factor family contains a basic helix-loop-helix (bHLH) motif consisting of 59 amino acids, which plays a wide range of regulatory role in plant growth and development, morphogenesis, and stress response. Orchid plants have important ornamental, cultural, and economic value due to their unique flower patterns, diverse colors, and rich leaf art. This article summarizes the structure and classification of TCP transcription factors, and reviews their functions in the morphogenesis of flowers, leaves, and other organs in orchids, laying a theoretical foundation for the study of TCP genes function in orchids. Reference | Related Articles | Metrics | Comments（0）

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Evaluation of Soil Heavy Metal Pollution in Maba Forest Park in Shaoguan and the Role of Different Silvicultural Modes in Its Remediation ZHANG Liang Subtropical Plant Science    2024, 53 (6): 560-568.   DOI: 10.3969/j.issn.1009-7791.2024.06.009 Abstract （32）      PDF（pc） （12289KB）（8）       Knowledge map    Save In order to investigate the long-term management of industrial polluted land by vegetation restoration mode and to continuously follow up the improvement effect of soil heavy metal pollution, this study took Maba Forest Park in Qujiang District, Shaoguan City as an example, chose the local standard of Soil Environment Background Value released in 2021 as the reference value, and evaluated the degree of its contamination by using the Nemero Comprehensive Pollution Index and the Potential Ecological Risk Index. The results showed that the average content of Cd exceeded the regional background value by 14.10 times, and the coefficient of variation of Cd reached 102%, indicating that Cd appeared to be enriched and seriously disturbed by the outside world, or related to the neighbouring Shaogan Expressway passing through, and the dumping of discarded stones; 90% of the sampling points reached the level of serious contamination; and the soil heavy metal contamination of the investigated area as a whole was divided into the categories of ‘slight’, ‘ecological risk’ and ‘potential ecological risk’. Soil heavy metal pollution in the survey area is mainly ‘slight’ and ‘very strong’ ecological risk, and Cd and Pb need to be paid attention to. Different afforestation modes affected soil heavy metal content, with artificial afforestation significantly reducing soil Cr content, thinning and renovation significantly reducing soil Cr and Ni content, and replanting treatments having a relatively mild effect. Reference | Related Articles | Metrics | Comments（0）

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Differential Analysis of Volatile Oil Composition of Notopterygii Rhizoma et Radix from Different Origins and Habitats ZHANG Jun, LI Qian Subtropical Plant Science    2025, 54 (1): 11-21.   DOI: 10.3969/j.issn.1009-7791.2025.01.002 Abstract （25）      PDF（pc） （1648KB）（170）       Knowledge map    Save The differences in volatile oil composition of Notopterygii Rhizoma et Radix (NRR) from different origins and habitats were studied to provide scientific basis for the quality control and resource development of NRR. Twenty batches of NRR herbs from different origins and habitats were used as experimental materials to extract the volatile oil by water vapour distillation. The volatile oil yields, compositions and relative contents of Notopterygium incisum (NI) and N. franchetii (NF) were systematically analyzed by GC-MS technology. The differences in chemical composition between NI and NF were interpreted from the perspective of volatile constituents by combining with cluster analysis (HCA), principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA). Results showed that the average volatile oil content of NI was higher than that of NF, whereas NF had a richer variety of volatile oil constituents. A total of 206 constituents were identified in the volatile oils from 20 batches, which were mainly hydrocarbons and alcohols. There were only 3 common constituents in 20 batches of the samples, which were all hydrocarbons. In addition, the 5 constituents with the highest contents, namely, β-pinene, (+)-α-pinene, (+)-limonene, p-mentha-1,4-diene, (-)-terpinen-4-ol were also significantly different in the different origins and habitats samples. The chemometrics analysis showed that HCA, PCA and OPLS-DA could effectively distinguish NI from NF, but could not be differentiated between samples of different habitats. A total of 23 differentiated volatile oil constituents were screened out using VIP > 1 as a judgement criterion, which can be used as an important constituent for distinguishing NI from NF. There were significant differences in the types and contents of the volatile oils of NRR from different origins, but there were no obvious differences among different habitats, indicating that origins are the dominant factors among the many factors that cause differences in the volatile compositions of NRR. The results of this study provide a theoretical basis for the the quality evaluation system of NRR. Reference | Related Articles | Metrics | Comments（0）

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Allelopathic Effect of Wedelia chinensis Fermented Broth on Microcystis aeruginosa LU Xiao-gui, XIE Qin-ming Subtropical Plant Science    2025, 54 (1): 29-36.   DOI: 10.3969/j.issn.1009-7791.2025.01.004 Abstract （25）      PDF（pc） （530KB）（7）       Knowledge map    Save The use of allelochemicals for the prevention and control of cyanobacterial blooms is considered to have the advantages of low cost and sustainable development, seeking the efficient and eco-friendly algaecides is crucial. In this experiment, Microcystis aeruginosa was chosen as the subject to explore the allelopathic effects of Wedelia chinensis fermented broth It was found that the overall allelopathic effect of the Wedelia chinensis fermented broth on Microcystis aeruginosa exhibited a phenomenon of "low promotion and high inhibition." Compared to the control group (CK), a low concentration (0.5 mg·mL–1) of the Wedelia chinensis fermented broth exhibited a maximum promotion effect of 17% on Microcystis aeruginosa growth, with cell densities of 8.73 × 105 cells·mL–1 in the CK group and 10.21 × 105 cells·mL–1 in the 0.5 mg·mL–1 treatment group. In contrast, the highest concentration (2.5 mg·mL–1) showed a maximum inhibition rate of 54%, with cell densities of 5.72 × 105 cells·mL–1 in the CK group and 2.61 × 105 cells·mL–1 in the 2.5 mg·mL–1 treatment group. After 10 days of treatment, the chlorophyll a content in M. aeruginosa significantly decreased, with the 2.5 mg·mL–1 experimental group showing chlorophyll levels significantly lower than those of the control group from the second day (P＜0.05), and by the tenth day, the chlorophyll content in the control group was 2.65 times that of the experimental group. Phycobiliproteins, which assist in photosynthesis, were found to be unaffected in their synthesis across different concentrations of pyrethrum Wedelia chinensis fermented broth compared to the control group (P＞0.05), the total protein of CK group and low to high concentration were 0.132, 0.134, 0.132, 0.129, 0.126, and 0.126 mg·L–1, respectively. On the tenth day, the effect of pyrethrum Wedelia chinensis fermented broth on the SOD content in M. aeruginosa generally showed an increasing trend. The SOD content in the control group was higher than in the 0.5 mg·mL–1 experimental group, but the difference was not significant (P＞0.05). In the experimental group with 2.5 mg·mL–1, the SOD content peaked at 2.25 U·mL–1, which was 2.1 times that of the control group. This study aimed to contribute foundational data for the prevention and control of cyanobacterial blooms. Reference | Related Articles | Metrics | Comments（0）

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Moliniopsis, a Newly Recorded Genus of Poaceae in Guangdong Province TONG Yi-hua, ZENG Yun-bao Subtropical Plant Science    2025, 54 (1): 85-88.   DOI: 10.3969/j.issn.1009-7791.2025.01.011 Abstract （25）      PDF（pc） （5695KB）（28）       Knowledge map    Save This paper reported a newly recorded genus of Poaceae in Guangdong, viz. Moliniopsis Hayata. This genus is monotypic with the type species, M. japonica (Hackel) Hayata, distributed in Russian Far East, Japan, South Korea and Southeast China (Anhui, Zhejiang, Jiangxi, Fujian). Recently, a population of this species was found in Conghua District, Guangzhou City, Guangdong Province, which is the southernmost population of this species. This new finding is of great significance for the future study of the geographical distribution and the origin and the spread of M. japonica. The vouchers are deposited in the herbarium of South China Botanical Garden, Chinese Academy of Sciences (IBSC). Reference | Related Articles | Metrics | Comments（0）

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Phenotypic Characteristics and Key Identification Points of Common Tabebuia and Handroanthus Species in Shenzhen ZHANG Xiao-feng, LUO Dong, YUAN Feng-jun, XU Gui-hong, TANG Jing-wen, LEI Jiang-li Subtropical Plant Science    2025, 54 (2): 195-200.   DOI: 10.3969/j.issn.1009-7791.2025.02.009 Abstract （24）      PDF（pc） （1109KB）（68）       Knowledge map    Save Tabebuia and Handroanthus (Bignoniacaea) species are famous woody plants in the world, and are widely cultivated in Shenzhen. However, some species are phenotypically similar, making them difficult to distinguish. Additionally, there is considerable confusion in the use of their Chinese names and Latin names. The article investigated and described in detail the types, phenotypes, and phenological characteristics of Tabebuia and Handroanthus species cultivated in the urban greenland from Shenzhen. It also conducts comparative analyses by reviewing relevant data and literature, aiming to provide scientific basis for the identification of phenotypic traits and germplasm evaluation of Tabebuia and Handroanthus species. The results showed that among the urban greenland in Shenzhen, six species from two genera (Handroanthus and Tabebuia) were primarily cultivated. Among these, there were three species with red flowers: Handroanthus impetiginosus, H. heptaphyllus, and Tabebuia rosea; and three species with yellow flowers: H. chrysanthus, H. chrysotrichus, and T. aurea. Among these species, H. impetiginosus and H. heptaphyllus, H. chrysanthus and H. chrysotrichus are extremely similar in flower color. However, there are differences in terms of leaf serration, petiole characteristics, calyx shape, and pubescence, which can be used as distinguishing traits between the species. Reference | Related Articles | Metrics | Comments（0）

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Subtropical Plant Science    2025, 54 (2): 240-.   Abstract （24）      PDF（pc） （3248KB）（8）       Knowledge map    Save Related Articles | Metrics | Comments（0）

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Species Diversity of Lycophytes and Ferns in Huidong County, Guangdong Province HUANG Jun-kai, LI Xiao-ji, DONG Shi-yong Subtropical Plant Science    2025, 54 (2): 186-194.   DOI: 10.3969/j.issn.1009-7791.2025.02.008 Abstract （23）      PDF（pc） （2106KB）（80）       Knowledge map    Save Huidong is a county of Guangdong Province in southern China, lying in the eastern region of the Guangdong-Hong Kong-Macao Greater Bay, and holds rich biodiversity. However, there is no checklist of lycophytes and ferns available for Huidong due to the lack of a comprehensive survey. Based on field surveys in recent years, the literature, herbarium specimens, and molecular phylogenetic analyses of some groups, we conducted crucial identifications of all specimens available to us from Huidong in order to document the species diversity and identify the floristic characteristics and rare and endangered species of lycophytes and ferns in this county. The main results include these five points: (1) New morphological forms were found in Selaginella heterostachys, Coryphopteris angulariloba, C. chingii, and Diplazium virescens. (2) Sphaerostephanos taiwanensis was confirmed with populations occurring in Guangdong, while Asplenium guangdongense was not supported to be an independent species from A. normale. (3) A population of Vandenboschia lofoushanensis, which had disappeared for nearly a century in the wild, was rediscovered in Huidong. (4) A total of 11 lycophyte species (belonging to 5 genera and 2 families) and 119 fern species (in 62 genera and 23 families) are recognized in Huidong. For ferns in this area, the Asia-Tropic distributing element and the East-Asian element are dominant, each accounting for about 36% species diversity, with relatively rich species in Polypodiaceae (17 spp.), Dryopteridaceae (15 spp.), and Thelypteridaceae (15 spp.). (5) Among lycophytes and ferns in Huidong, thirteen species are rare and/or endangered; eight species hold Class II national protection status in China, with Huperzia javanica and V. lofoushanensis accessed as endangered (EN) under IUCN criteria. A complete list of lycophytes and ferns in Huidong, along with the information on the distribution pattern for each taxon, is provided. Reference | Related Articles | Metrics | Comments（0）