Effects of Exogenous Strigolactone on Axillary Bud Elongation and Expression of Genes Related to the 
Strigolactone Metabolic Pathway in Tobacco

doi:10.3969/j.issn.1009-7791.2023.05.001

Subtropical Plant Science ›› 2023, Vol. 52 ›› Issue (5): 369-380.DOI: 10.3969/j.issn.1009-7791.2023.05.001

• Plant physiology, biochemistry and molecular biology • Previous Articles Next Articles

Effects of Exogenous Strigolactone on Axillary Bud Elongation and Expression of Genes Related to the Strigolactone Metabolic Pathway in Tobacco

TIAN Hui-yuan, TANG Bo-xi, WANG Yuan-xiu, LIU Fan, GUO Kai-yang, LIU Guo-qin^*

(College of Tobacco Science, Guizhou University / Guizhou Key Laboratory for Tobacco Quality Research, Guiyang 550025, Guizhou China)

Received:2023-06-27 Accepted:2023-09-03 Online:2023-10-30 Published:2024-01-19
Contact: LIU Guo-qin

外源独脚金内酯对烟草腋芽伸长及独脚金内酯代谢途径相关基因表达的影响

田慧源，唐博希，王原秀，刘帆，郭凯阳，刘国琴^*

(贵州大学烟草学院 / 贵州省烟草品质研究重点实验室，贵州贵阳 550025)

通讯作者: 刘国琴
基金资助:
国家自然科学基金(32060420)

Abstract

Abstract: The effects of strigolactone (SL) on the elongation of tobacco axillary buds and the expression of genes related to its metabolic pathway were carried out, to explore the relationship between SL and the growth of tobacco axillary buds and the expression changes of SL-related genes during the elongation of tobacco axillary buds, which could establish a theoretical basis for the molecular regulation mechanism of SL in regulating the growth of tobacco axillary buds. This study investigated the developmental changes of tobacco axillary meristematic tissues after decapitation, and observed the various genes associated with SL metabolic pathway of tobacco axillary buds by treatment with GR24, an artificial synthetic analogue of SLs, using paraffin sectioning and transcriptome technique. The results showed that the axillary meristem of tobacco was composed of three cell regions: central blast region (CM), peripheral meristem (PM) and costal meristem (RM), among which the CM was located at the top of the axillary meristem, which could form PM and RM through the division of basal and lateral cells, the PM was located on both sides of the axillary meristem, and the RM was located below the CM region and the inner side of the PM region. The cell volume and arrangement of the three cell regions were different, indicating that there were differences in cell division in each part of the meristem. After axillary meristem development to form axillary buds, it was found that GR24 could inhibit the elongation of axillary buds at 1–3 nodes by exogenous application of different plant growth regulators, while BR could promote the elongation of axillary buds. Transcriptome analysis revealed that SL could affect the expression of genes related to its own metabolic pathway, SL induced the expression of D27, D14, DAD2 and SMAX1-LIKE4 compared to CK and TIS-108 [a triazole-type strigolactone(SL)-biosynthesis inhibitor] treatment, whereas, D27, D14 and DAD2 were down-regulated by TIS-108 treatment, the expression of SMAX1 was inhibited by SL, while TIS-108 treatment induced the expression of SMAX1. The axillary buds of tobacco are developed from three hyphal regions of the axillary meristem, while different plant growth regulators have different effects on the axillary buds of tobacco. BR can promote the elongation of the axillary buds of tobacco, while SL can inhibit the elongation of the axillary buds, and the genes related to the metabolic pathway may play an important regulatory role in the elongation of the axillary buds of tobacco.

Key words: strigolactone, tobacco, axillary buds, meristem, gene expression

摘要： 通过研究外源独脚金内酯(SL)对烟草Nicotiana tabacum腋芽伸长及其SL代谢途径相关基因表达的影响，探究SL与烟草腋芽生长的关系及烟草腋芽伸长过程中SL相关基因的表达变化，为SL调控烟草腋芽生长的分子机理研究提供理论依据。采用石蜡切片和转录组技术研究烟株打顶后烟草腋芽分生组织发育及在SL生物合成类似物GR24处理下腋芽SL代谢途径相关基因表达的变化。结果表明，烟草腋分生组织由中央母细胞区(CM)、周缘分生组织(PM)和肋状分生组织(RM)三个细胞区域组成，其中CM位于腋分生组织顶端，其可通过基部及侧面细胞的分裂形成PM及RM，PM位于腋分生组织两侧，RM处于CM下方及PM内侧的位置。三个细胞区域的细胞体积、排列方式不同，说明分生组织中各部分细胞分裂存在差异性；腋分生组织发育形成腋芽后，通过外源施加不同植物生长调节剂发现，GR24能抑制1～3节位腋芽的伸长，而BR能促进腋芽伸长；转录组分析发现，SL能影响其自身代谢途径相关基因的表达，与CK和独脚金内酯合成抑制剂(TIS-108)处理相比，SL诱导D27、D14、DAD2和SMAX1-LIKE4的表达，而在TIS-108处理中D27、D14及DAD2下调表达，SMAX1的表达受到SL的抑制，而TIS-108处理诱导SMAX1的表达。烟草腋芽由腋分生组织三个细胞区域发育而来，同时，不同植物生长调节剂对烟草腋芽的影响不同，BR促进烟草腋芽的伸长，而SL抑制腋芽伸长，且在烟草腋芽伸长过程中其代谢途径相关基因可能发挥着重要的调控作用。

关键词: 独脚金内酯, 烟草, 腋芽, 分生组织, 基因表达

CLC Number:

Q944

TIAN Hui-yuan, TANG Bo-xi, WANG Yuan-xiu, LIU Fan, GUO Kai-yang, LIU Guo-qin. Effects of Exogenous Strigolactone on Axillary Bud Elongation and Expression of Genes Related to the Strigolactone Metabolic Pathway in Tobacco[J]. Subtropical Plant Science, 2023, 52(5): 369-380.

田慧源, 唐博希, 王原秀, 刘帆, 郭凯阳, 刘国琴. 外源独脚金内酯对烟草腋芽伸长及独脚金内酯代谢途径相关基因表达的影响[J]. 亚热带植物科学, 2023, 52(5): 369-380.

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Effects of Exogenous Strigolactone on Axillary Bud Elongation and Expression of Genes Related to the Strigolactone Metabolic Pathway in Tobacco

外源独脚金内酯对烟草腋芽伸长及独脚金内酯代谢途径相关基因表达的影响

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