Abstract: Cutting is one of the important methods to propagate and preserve good varieties and resources, especially for self-incompatibility and cross-pollination alfalfa. Currently, there are some limitations in the application of current alfalfa cutting methods, such as complicated operation and long cycle. Based on the theory of root regeneration controlled by mitochondria, a simple and efficient new cutting method of alfalfa, named semi-wet cutting method was proposed in this study. This method can be completed only by covering the cuttings with moisturizing material and placing them in a container. In this experiment, two alfalfa varieties, ‘Zhongmu No. 1’ and ‘Xinjiang Daye’, were selected and cultured by semi-wet method and hydroponics method, aiming at comparing the rooting effect of different varieties under semi-wet method and the difference between the two cutting methods. The experimental results showed that there was no significant difference in the rooting rate of the two varieties by using semi-wet cutting, and the rooting rate was very high, more than 90% on the 8th day and close to 100% on the 10th day in both varieties, which was significantly faster than that by using hydroponic cutting. To sum up, semi-wet cutting not only simplified the operation process, but also effectively shortened the rooting time, which has important practical application value for the propagation of alfalfa and the collection of field resources.

Key words: semi-wet method; alfalfa cutting; rooting rate

摘要： 扦插是良种扩繁及资源保存的重要手段之一，对于自交不亲和、异花授粉的紫花苜蓿来说尤其重要。目前已有的苜蓿扦插方法在应用中还存在一定的局限性，如操作复杂、周期较长等问题。本研究依据线粒体调控根再生理论提出了一种简便高效的新型苜蓿扦插方法—半湿法扦插。本实验选取了‘中苜一号’与‘新疆大叶’两种常用紫花苜蓿品种，用保湿材料将插穗覆盖后放置于简易容器中，同时评估了半湿法与水培法扦插下的生根效果及两种扦插方法的差异。结果表明，半湿法扦插在两个不同品种背景下的生根率没有明显差异，生根效率都非常高，第8天生根率超过90%，到第10天接近100%，且显著高于水培扦插。综上所述，半湿法扦插不仅简化了扦插的操作流程，还有效地缩短了生根时间，对苜蓿的扩繁及野外资源采集具有重要的实际应用价值。

关键词: 半湿法；苜蓿扦插；生根率