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    10 March 1991, Volume 20 Issue 01 Previous Issue    Next Issue

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    INHIBITION OF CALCIUM ON ETHYLENE PRODUCTION IN POSTHARVEST BANANA FRUITS
    Chen Xiaoming, Huang Weinan, Lu Rongxin, Zhuang Rongfu
    1991, 20(01): 1-5. 
    Abstract ( )   PDF (339KB) ( )  
    Banana (Musa acuminata cv. ‘Dwarf Davendish’) fruit tissues were vacuum infiltrated with CaCl2 solutions in order to determine the influence of calcium upon ethylene production, EFE activity, ACC content and ACC/MACC ratio. The results showed that calcium treatment inhibited ethylene production in both peel and pulp, particularly in peel. Ethylene production in peel treated with 3 concentrations of CaCl2 solutions (0.2, 0.6 and 0.8 mol/L) remained lower than that in the control throughout the period of 2-6 hours after treatment.Ethylene production in pulp infiltrated with 0.2 and 0.6 mol/L CaCl2 were inhibited during 2-3 hours after treatment,and ethylene production was reduced by 0.8 mol/L CaCl2 treatment throughout 2-6 hours (Fig. 1). Calcium infiltration (with 0,2, 0.4 and 1.0 mol/L CaCl2) lowered the internal ACC level in both peel and pulp (Tab. 1).EFE activity in peel and pulp treated with 0.05, 0.1, 0.2 and 0.5 mol/L CaCl2 were reduced (Tab. 2). The result also showed that calcium treatment contributed to the change of ACC/MACC ratio (Fig. 2). The results suggest that the inhibition of Ca2+ on ethylene production in banana tissues, does not merely act on the step of ACC to ethylene, Ca2+ may also regulate both ACC biosyn thesis and the bioconversion of ACC to MACC.
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    AMMONIA ASSIMILATION IN Rhodobacter sphaeroides
    Zou Xiaolu, Song Hongyu
    1991, 20(01): 6-11. 
    Abstract ( )   PDF (382KB) ( )  
    The activities of glutamine synthetase (GS), glutamate syn-thase (GOGAT), glutamate dehydrogenase (GDH) and alanine dehydro-genase (ADH) were detected in crude extracts of Rhodobacter sphaeroides strain 601. The GDH activity of the strain in cultures grown at low concentration of ammonia was low, but high when an excess of ammonia was present. On the other hand, GS/GOGAT activities were usually higher when the ammonia concentration was limited for growth. Addition of methionine sulphoximine (MSX), an inhibitor of GS, to strain 601 growing on ammonia or molecular nitrogen as nitrogen source resulted in cessation of growth, which did not occur when the inhibitor was added to strain 601 growing on L-glutamate. The results demonstrated that ammonia was assimilated via GS/GOGAT pathway and the GDH pathway in Rhodobaeter sphaeroides strain 601.
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    EFFECTS OF PLANT REGULATORS ON KEEPING FRUITS ON TREE AND STORAGE LIFE OF JINCHENG ORANGE
    Liu Xiaodong, Shen Zhacxming, Zhao Xueyuan, M. D'. Amico, P. L. Trigle, S. Torrisi, F. Gorini
    1991, 20(01): 12-17. 
    Abstract ( )   PDF (386KB) ( )  
    When the rind color of Chincheng oranges turned from dark green to light green in mid and late September, spraying the fruits with 15~30 ppm GA+50 ppm 2,4-D continuously 2 to 3 times at one-month intervals delayed the maturity of the fruits.Keeping the fruits on tree for 2~3 months, the fruit retention rate was 93%, with bright rind color and high soluble solid content. The pulp was delicate, flavorous and sweet. The treatment also prolonged the harvest time from mid November to late December and storage life of the fruits was extended to late Apri the next year. The percentage of decayed and drought spot fruits wast low.
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    A PRELIMINARY STUDY ON ECOLOGICAL DISTRIBUTION OF SOIL MICROORGANISM UNDER DIFFERENT ORIGINAL Castanopsis kawakamii STANDS
    Li Jiahe, Li Jinfeng
    1991, 20(01): 18-23. 
    Abstract ( )   PDF (390KB) ( )  
    The ecological distribution of soil microorganism under the natural and artificial original Castanopsis kawakamii stands was studied.The quantity of bacterium is greater than that of all other microorganisms under both stands, the proportion of Baciluss/bacterium under artificial stand ismuch greater than that under natural stand, the total microbe, bacterium,fungi and azotobacter under the natural stand are greater than those under artiftcial stand. Penicillium and Trichoderma are the dominant species over fungi, and Flavus and Albosporus are the main compositions of actinomyces, but the quantity of them under the artificial stand is greater than those under the natural stand.
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    LIQUID FERMENTATION CONDITIONS FOR Ganoderma lucidum
    Liu Sinmin, Lin Yaohui, Xu Wenjiang
    1991, 20(01): 24-28. 
    Abstract ( )   PDF (309KB) ( )  
    Some factors influencing liquid fermentation of Ganoderma lucidum were examined. The experiments showed that 4-day old spawn and 60 ml of culture medium in 300 ml shake-bottle gave the best fermentation result. Sucrose and peanut meal were ideal carbon source and nitrogen source, respectively. The optimum formula of culture medium selected was. sucrose 4%, peanut meal 3%, (NH4)2SO4 0.15%, KH2PO4 0.15%. The dry weight of mycelium and polysaccharide were up to 0.83 g and 0.3 g per 100 ml fermented liquid, respectively. These results provide the basis for increasing yield and active constituents of fermented pro-duct of Ganoderma lucidum.
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    SCANNING ELECTRON MICROSCOPIC OBSERVATION ON SEED GERMINATION OF Vanda amesia AND Cymbidium floribundum
    Lu Xuehua, Lin Hanzhang
    1991, 20(01): 29-34. 
    Abstract ( )   PDF (2256KB) ( )  
    The in vitro morphological changes during seed germination of Vanda amesia and Cymbidium floribundum were examined by scanning elect-on microscope. During Vanda amesia seed germination, the embryo was genfrally referred as the "protocorm" and was visible to the naked eyes. The germination of Cymbidium floribundum resembled to that of Vanda amesia except that rhizome was formed after germination and leaf development was poorer. Leaves assumed scumed scale-shape and tightly covered the meristem of the rhizome. Photos of scanning electron microscope revealed that each hair was an outgrowth of a papila protruding out from the outer wall of the epidermal cfll, thus equivalens to a roothair.
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    NODULATION AND NITROGEN FIXATION CHARACTERISTICS OF Sesbania cannabina
    Cai Keqiang, Huang Weinan
    1991, 20(01): 35-39. 
    Abstract ( )   PDF (341KB) ( )  
    Sesbania cannabina interplanted in orchard as summer green manure appeared nodules after 10-15 days' growth. Their taproots and lateral roots developed abundant nodules. The total amount of nodules was estimated to be 30 kg per mu. The nitrogen fixiug activity,as determined by acetylene reduction assay, reached a maximum at vegetative period, and declined during flowering and podding stages because the taproot nodules began to rot. Soil drought reduced moisture content and nitrogenase activity of nodules. The optimum temperature for nitrogen fixation of nodules was 25℃ and the nitrogenase activity markedly decreased below 12℃ or over 35℃. Approximately 7.6 kg nitrogen per mu was fixed by nodulated S.can-nabina during vegetative period under our experimental conditions.
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    EFFECTS OF PRETRE ATMENT WITH CHEMICALS ON FRESH PRESERVATION OF BUT CUT FREESJA FLOWERS
    Chen Shilin, Huang Minting
    1991, 20(01): 40-44. 
    Abstract ( )   PDF (339KB) ( )  
    The effects of pretreatment with chemicals on fresh preservation of bud cut freesia flowers were studied. The results showed that prstreatmant improved ornamsatal quality and prolonged vase life to different extent.The best treatment was 8 HQC+STS+BA+Sucrose, which increased opening by 37.6% and flower diameter by 5.6 mm and prolonged vase life for 6.9 days, compared to the control. The results also indicated that storability was obviously improved. Stored at 4℃ for 5 days, the ornamental quality of cut freesia flowers pretreated with chemicals was markedly higher and the vase life was longer as compared with the control. Storability varied with different varieties. We come to the conclusion that the increase of the rate of absorption and transpiration and the prolongation of the time of water balance are the important reasons why pretreatment improves ornamental quality and prolongs vase life of cut flowers.
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    EFFECT OF leucaena leucocephala LEAVES ON THE HYPHAE GROWTH OF EDIBLE MUSHROOMS AND DEGRADATION OF MIMOSINE BY HYPHAE
    Liu Fuping, Chen Fangshun, Huang Weinan
    1991, 20(01): 45-49. 
    Abstract ( )   PDF (342KB) ( )  
    Leacaena leueocephola leaves used as nitrogenous additive in media accelerated the hyphae growth of Pleurotus florida, Auricularia auricula and Lgntinus edcdes.Mimosine degraded automatically and notably during the hyphae growth in raw substrate and was destroyed heavily with autoclave. In addition, mimosine was degraded remarkably by these hyphae.
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    APPLICATION OF PLANT HORMONES IN CITRUS SEEDLING PRODUCTION
    Zhang Jicren, Gao Feng
    1991, 20(01): 50-54. 
    Abstract ( )   PDF (425KB) ( )  
    In this paper, the effects of plant hormones on germination of rootstock seeds, graft survival, growth of seedlings and rooting of cuttings in citrus seedling production are outlined.
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    KUDINGCHA——MEDICINAL PLANTS NATIVE TO FUJIAN PROVINCE
    Chang Yongtian
    1991, 20(01): 55-57. 
    Abstract ( )   PDF (1029KB) ( )  
    The confusion around the name KUDINGCHA is discussed and the concept of this name is treated here. Ilex latifolia Thunb., native to Fujian, is used for KUDINGCHA with more detailed accounts.
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