关键词: 香果树, 植物生长抑制剂, 种质, 离体保存

Abstract: The stem segments with buds of Emmenopterys henryi were used as explants for studying the effects of 5 plant growth inhibitors at different temperature and light conditions on the in vitro conservation by single-factor experimental method.The results showed that at 25℃,the best CCC,MH,B₉,S3307 and ABA concentrations of Emmenopterys henryi germplasm in vitro conservation were 4 mg/L,4 mg/L,2 mg/L,1 mg/L and 4 mg/L.At 9℃,the best CCC,MH,B₉,S3307 and ABA concentrations were 2 mg/L,1 mg/L,1 mg/L,0.5 mg/L,and 2 mg/L.On light or dark conditions,the best CCC,MH,B₉,S3307 and ABA concentrations were 4 mg/L,4 mg/L,2 mg/L,1 mg/L,and 4 mg/L.Compared with normal temperature conditions,5 plant growth inhibitors had higher survival rate and their best concentrations for Emmenopterys henryi germplasm in vitro conservation generally reduced on low-temperature conditions.And compared with light conditions,5 plant growth inhibitors also had higher survival rate on dark conditions,but their best concentrations were the same as light conditions.The genetic variation of Emmenopterys henryi in vitro conservation plantlets did not appear.

Key words: Emmenopterys henryi, plant growth inhibitor, germplasm, in vitro conservation