亚热带植物科学 ›› 2012, Vol. 41 ›› Issue (01): 48-50.DOI: 10.3969/j.issn.1009-7791.2012.01.012

• 研究报告 • 上一篇    下一篇

梳唇石斛试管开花诱导和离体保存

赵大克,李春芳,程治英,杨俊波   

  1. 中国科学院 昆明植物研究所 西南野生生物种质资源库,云南 昆明 650204
  • 收稿日期:2011-12-07 修回日期:2011-12-07 出版日期:2012-03-10 发布日期:2012-03-10
  • 通讯作者: 杨俊波
  • 作者简介:赵大克(1983-),男,山东聊城人,博士研究生,从事保护生物学研究。
  • 基金资助:

    中国西南野生生物种质源库项目(0802261411)

In vitro Flowering and Conservation of Dendrobidium strongylanthum Rchb.f.

ZHAO Da-ke,LI Chun-fang,CHEN Zhi-ying,YANG Jun-bo   

  1. China Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of sciences, Kunming 650204, Yunnan China
  • Received:2011-12-07 Revised:2011-12-07 Online:2012-03-10 Published:2012-03-10
  • Contact: YANG Jun-bo

摘要: 对珍稀濒危药用植物梳唇石斛(Dendrobidium strongylanthum Rchb. f.)离体开花诱导和保存进行试验、观察和分析,研究基本培养基和植物生长调节剂对诱导离体开花的影响以及培养条件对离体保存的影响。结果显示,试管丛芽培养在MS+6-BA 2 mg/L+NAA 0.2 mg/L的培养基上,培养20 d试管开花的诱导率高达96%以上。试管丛芽接种在花宝1号3 g/L+NAA 1 mg/L+活性炭0.5 g/L培养基上,在12 ℃和HR 60%条件下保存,继代周期可延长至2年。该物种快速繁殖方法和保存技术的成功,为濒危植物梳唇石斛持续利用提供了技术支持。

关键词: 梳唇石斛, 丛芽, 开花诱导, 离体保存

Abstract: The In vitro flowering and conservation of Dendrobidium strongylanthum were conducted, observed and analyzed. The impact of medium and plant hormones on in vitro flowering and the influence of environment (at 12 ℃, HR 60%) on in vitro conservation were also studied. The results showed the induction rate of cluster buds in MS+6-BA 2 mg/L+NAA 0.2 mg/L with 3% Sucrose for 70 d was over 96%. Moreover, the cluster buds could be inoculated in hyponex 1# (C:P:K=7:6:19) 3 g/L+NAA 1 mg/L+AC 0.5 g/L with 2% Sucrose at 12 ℃, HR 60% to make subculture cycle for two years. The success of rapid propagation and In vitro conservation provided technical support for the sustainable utilization of Dendrobidium strongylanthum.

Key words: Dendrobidium strongylanthum, clustar bud, induction of flowering, in vitro conservation

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