关键词: 兰属, 种间杂交, 胚拯救

Abstract: Interspecific hybridization was carried out with Cymbidium hybridium as female parent and C. sinense as male parent.Embryo rescue technique of hybrids was also studied.The results showed that ovaries culture was better than ovules culture, and ovaries culture had become to be effective means of embryo rescue.The germination and growth had significant difference at different growth stages and different culture medium.Ovaries could be cultured in vitro successfully as early as 60d after pollination.However, the germination rate was the highest when ovaries cultured for 90d after pollination, which reached up to 80%.Of the media studied, VW+BA 0.2 mg/L+NAA 0.2 mg/L+peptone 3 g/L+cascin hydrolyaate 500 mg/L+coconut milk 10% was the optimal medium for ovaries culture.

Key words: Cymbidium, interspecific hybridization, embryo rescue