红掌茎段侧芽离体快繁技术研究

doi:10.3969/j.issn.1009-7791.2011.02.013

›› 2011, Vol. 40 ›› Issue (02): 42-43.DOI: 10.3969/j.issn.1009-7791.2011.02.013

红掌茎段侧芽离体快繁技术研究

陈丽文,何贵整

（钦州市林业科学研究所，广西钦州 535000）

收稿日期:2011-02-21 修回日期:2011-02-21 出版日期:2011-06-10 发布日期:2011-06-10
通讯作者: 陈丽文
作者简介:陈丽文(1980-)，女，广西苍梧人，工程师，从事林木、花卉组培快繁技术研究。

Technique for in vitro Rapid Propagation of Stem Buds of Anthurium andraeanum

CHEN Li-wen,HE Gui-zheng

(Qinzhou Forestry Research Institute, Qinzhou 535000, Guangxi China)

Received:2011-02-21 Revised:2011-02-21 Online:2011-06-10 Published:2011-06-10
Contact: CHEN Li-wen

摘要/Abstract

摘要： 以红掌嫩茎为外植体，诱导侧芽萌发，并进行增殖和生根培养，研究不同生长调节剂浓度配比对茎段侧芽萌发、增殖、无菌苗生根的影响以及增殖培养过程中愈伤组织的抑制等因素。结果表明，侧芽诱导的适宜培养基为MS + 6-BA 2.0 mg/L + NAA 0.5 mg/L，萌发率达87.5%；最适增殖培养基为MS + 6-BA 0.8 mg/L + NAA 0.2 mg/L + VB2 8.0 mg/L，增殖系数3.8；最适生根培养基为1/2MS + NAA 0.5 mg/L，生根率98%；在增殖培养基中添加适量VB2能较好地抑制愈伤组织的生成，防止愈伤组分织分化形成芽，从而达到以芽繁芽的目的。

关键词: 红掌, 茎段侧芽, 离体快繁

Abstract: The tender stem of Anthurium andraeanum was used as explants to study the effects of different growth regulators concentrations on the stem bud's germination, proliferation, rooting culture and the prevention of callus during proliferation culture. The results indicated that the optimal culture medium for stem bud inducement was MS + 6-BA 2.0 mg/L + NAA 0.5 mg/L, with the germination rate was 87.5%；the optimal culture medium for proliferation was MS + 6-BA0.8 mg/L + NAA 0.2 mg/L + VB2 8.0 mg/L, with the multiplication coefficient was 3.8; the optimal culture medium for rooting was 1/2MS + NAA0.5 mg/L, with the rooting rate was 98%; in addition, appropriate VB2 in proliferation medium could efficaciously prevent the form and differentiation of callus, thus reaching the goal that propagating buds by buds.

Key words: Anthurium andraeanum, stem bud, in vitro rapid propagation

中图分类号:

Q943.1

陈丽文,何贵整. 红掌茎段侧芽离体快繁技术研究[J]. , 2011, 40(02): 42-43.

CHEN Li-wen,HE Gui-zheng. Technique for in vitro Rapid Propagation of Stem Buds of Anthurium andraeanum[J]. , 2011, 40(02): 42-43.

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红掌茎段侧芽离体快繁技术研究

Technique for in vitro Rapid Propagation of Stem Buds of Anthurium andraeanum

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