雷公藤离体快繁过程中内源激素水平的变化

doi:10.3969/j.issn.1009-7791.2016.01.007

›› 2016, Vol. 45 ›› Issue (01): 32-37.DOI: 10.3969/j.issn.1009-7791.2016.01.007

• 植物生理生化与分子生物学 • 上一篇下一篇

雷公藤离体快繁过程中内源激素水平的变化

黄淑燕,郑郁善

(福建林业职业技术学院，福建南平 353000)

收稿日期:2015-11-26 修回日期:2016-04-17 出版日期:2016-03-10 发布日期:2016-03-10
通讯作者: 郑郁善
作者简介:黄淑燕，硕士，从事园林植物组织培养和中药材GAP研究。
基金资助:
国家科技支撑计划(2009BAI73B00)；福建省科技重大专项(2004YZ02-05)；福建省中药材GAP工程技术研究中心(2008Y2001)

Changes in Levels of Endogenous Plant Hormones During in vitro Rapid Propagation of Tripterygium wilfordii

HUANG Shu-yan,ZHENG Yu-shan

(Fujian Forestry Vocational Technical College, Nanping 353000, Fujian China)

Received:2015-11-26 Revised:2016-04-17 Online:2016-03-10 Published:2016-03-10
Contact: ZHENG Yu-shan

摘要/Abstract

摘要： 对雷公藤Tripterygium wilfordii组培苗各生长发育阶段的内源激素变化进行研究。结果表明，在芽诱导过程中，培养7～14 d是芽诱导的关键性阶段，ZT含量呈上升趋势，在培养28～35 d时，ABA含量转为上升，芽体生长进入缓慢抑制阶段；在芽继代增殖过程中，GA3含量升高，ABA含量降低，GA3/IAA比值高及ABA/IAA比值低利于雷公藤芽体分化增殖；在壮苗培养过程中，IAA、GA3含量上升，但两者浓度过高或过低均不利于生长；生根培养第7 天，雷公藤根系开始生成，IAA含量达到一峰值，在生根初期，低比值ZT/IAA及高比值GA3/ABA利于雷公藤生根培养。

关键词: 雷公藤, 离体快繁, 内源激素

Abstract: The dynamic changes of endogenous plant hormones from in vitro seedlings of Tripterygium wilfordii were determined. The results showed that the culture of 7—14 d is the key stage of bud induction. It has been basically completed in 21 d. Lower concentrations of GA3 is conductive to the growth and differentiation of buds. In the training of 28—35 d, the bud growth began to enter the stage of slow inhibition; in the process of bud subculture, we should properly control the culture time, timely transfer, reduce the content of the auxin and increase the content of auxin. At the same time, high ratio of GA3/IAA and low ratio of ABA/IAA is good for the bud differentiation and proliferation of T. wilfordii; in seedling cultivation process, higher levels of auxin and gibberellin were needed. After the culture of 14 d, the medium should be replaced in time. In the culture of 7th day, the roots begin to grow. In the early stage of root formation, low concentration of ABA is favorable for the formation of callus at the cut point. At the early stage of root development, the low ratio of ZT/IAA and high ratio of GA3/ABA were favorable to the rooting culture of T. wilfordii.

Key words: Tripterygium wilfordii, in vitro rapid propagation, endogenous hormone

中图分类号:

Q946.885

黄淑燕,郑郁善. 雷公藤离体快繁过程中内源激素水平的变化[J]. , 2016, 45(01): 32-37.

HUANG Shu-yan,ZHENG Yu-shan. Changes in Levels of Endogenous Plant Hormones During in vitro Rapid Propagation of Tripterygium wilfordii[J]. , 2016, 45(01): 32-37.

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雷公藤离体快繁过程中内源激素水平的变化

Changes in Levels of Endogenous Plant Hormones During in vitro Rapid Propagation of Tripterygium wilfordii

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