关键词: 华南十大功劳, 提取物, 抑菌活性, 不同溶剂, 索氏提取, 超声提取

Abstract: Through the bacteriostatic test of extract from Mahonia japonica, one of medical plants. The result are as follows. Different substances were extracted with different extracting methods of Ultrasonic and Soxhlet in different solution and in different polar solvent. To get the antibacterial and antifungal activity by suppression of bacterial ring method and including poison of medium. The antimicrobial substance exists in the stems and leaves, and leaves is obvious; there is a little difference on extracting methods of Ultrasonic and Soxhlet. The minimal inhibitory concentration of the extract on Staphylococcus aureus, Escherichia coli, Bacillus subtilis，Micrococcus luteus, Monilia albicans are 0.6 g/mL, 1.0 g/mL, 0.8 g/mL, 1.0 g/mL, 2.0 g/mL respectively; the minimal bactericidal concentration all are 2.0 g/mL; but it is ineffective on Aspergillus niger;the optimum antibacterial effect of the solvent should be Methanol. The bacteriostatic test by different polar solvent shows that the antibacterial substance easily dissolved in nonpolar solvent such as petroleum ether, ethyl ether. The bacterial and fungal of antimicrobial experiment have a wide disparity in sensibility as follows: S. aureus＞B. subtilis＞M. luteus＞E. coli＞M. albicans，and it is no sensitive to Aspergillus niger.

Key words: Mahonia japonica, extract, antimicrobial activity, different solvent, Soxhlet extraction, ultrasonic extraction