›› 2013, Vol. 42 ›› Issue (03): 199-204.DOI: 10.3969/j.issn.1009-7791.2013.03.003

• 研究报告 • 上一篇    下一篇

墨旱莲提取物抑菌活性初步研究

欧阳蒲月,梁永枢,莫小路,陈功锡,沈龙强   

  1. (1.广东食品药品职业学院,广东 广州 510520;2.广东省中药研究所,广东 广州 510520;3.吉首大学 植物资源保护与利用湖南省高校重点实验室,湖南 吉首 416000)
  • 收稿日期:2013-03-12 修回日期:2013-03-12 出版日期:2013-09-01 发布日期:2013-09-01
  • 通讯作者: 莫小路
  • 作者简介:欧阳蒲月(1978-),女,湖南邵阳人,讲师,硕士,从事药用植物学教学与研究工作。E-mail: ouyangpy@gdyzy.edu.cn
  • 基金资助:
    广东省科技厅粤港关键领域重点突破项目(2009A030901011)、植物资源保护与利用湖南省高校重点实验室开放项目(JZ200906)

The Preliminary Research of Antibacterial and Antifungal Activity of Extract from Eclipta prostrata

OUYANG Pu-yue,Liang Yong-shu,MO Xiao-lu,CHEN Gong-xi,SHENG Long-qiang   

  1. (1.Guangdong Food and Drug Vocational College, Guangzhou 510520, Guangdong China;2.Guangdong Research Institute of Traditional Chinese Medicine, Guangzhou 510520, Guangdong China; 3.Key Laboratory of Plant Resource Conservation and Utilization, Jishou University, Jishou 416000, Hunan China)
  • Received:2013-03-12 Revised:2013-03-12 Online:2013-09-01 Published:2013-09-01
  • Contact: MO Xiao-lu

摘要: 对药用植物墨旱莲Eclipta prostrata提取物的抑菌活性物质进行研究。结果表明:(1)通过不同部位提取物的抑菌试验,发现只有叶和根茎含有抑菌活性物质;(2)墨旱莲提取物对金黄色葡萄球菌、大肠杆菌、枯草杆菌、藤黄球菌的最低抑菌浓度分别为0.6、2.0、0.8、1.0 g/mL,最小杀菌浓度均为1.0 g/mL,对白色念珠菌有很强的抑菌作用,但对黑曲霉无效;(3)通过不同极性溶剂的抑菌试验表明,抑细菌活性物质易溶于石油醚、乙醚等非极性溶剂,具有最佳抑细菌效果的提取溶剂为偏碱性的65%乙醇溶液;(4)墨旱莲的抑菌活性物质可能为黄酮类物质。

关键词: 墨旱莲, 提取物, 抑菌活性, 溶剂, 索氏提取, 超声提取

Abstract: Through the bacteriostatic test of extract from Eclipta prostrata, one of medical plants in the west of Hunan, the results are as follows. (1) The antibacterial substance only exists in the petiole and rhizome. (2) The minimal inhibitory concentration of the extract on Staphylococcus aureus, Escheichia coli, Bacillus subtilis, Micrococcus luteus are 0.6, 2.0, 0.8, 1.0 g/mL respectively; the minimal bactericidal concentration all are 1.0 g/mL; but it is ineffective on Aspergillus niger. (3) In order to get the extract that has the optimum antibacterial effect, the solvent should be alkaline 65% ethyl alcohol. The bacteriostatic test by different polar solvent shows that the antibacterial substance easily dissolved in non-polar solvent such as petroleum ether, ethyl ether. (4) The extract may be flavone substance.

Key words: Eclipta prostrate, extract, antibacterial activity, solvent, soxhlet extraction, ultrasonic extraction

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