关键词: 榕属, 斑马鱼, 急性毒性, 闽南中草药

Abstract: Zebrafish model was used to evaluate the acute toxicity of aqueous extracts and alcohol extracts of different medicinal parts of Ficus hirta, F. erecta var. beecheyana, F. pumila, F. variolosa. The mortality rate of fish was recorded at 48 h and 96 h after the administration of the extracts, and the embryonic malformation rate and embryonic development of each group were observed by stereomicroscopy. The results showed that the water extracts of the roots, stems, leaves, fruits and other different medicinal parts of the plants of Ficus in Moraceae were low or non-toxic at a dose of 200 μg·mL^-1, and had no adverse effects on zebrafish embryos, the effect of promoting embryo growth was observed. However, the alcohol extract had a certain effect on zebrafish embryo development at high doses. The alcohol extracts from thick leaf of F. hirta, the stems and leaves of F. erecta var. beecheyana, and the leaves of F. variolosa led to the tail deformity of zebrafish embryos. The alcohol extracts from F. pumila leaves, fruit had obvious pericardial edema. The results showed that the above-mentioned medicinal materials were safe and non-toxic when used as traditional soup materials, but the dosage should be paid attention to when used as medicinal wine raw materials.

Key words: Ficus, zebrafish, acute toxicity, Minnan Chinese herbs