亚热带植物科学 ›› 2020, Vol. 49 ›› Issue (06): 469-472.DOI: 10.3969/j.issn.1009-7791.2020.06.011

• 植物资源与分类 • 上一篇    下一篇

二维液相色谱识别黄果野鸦椿及其原变种

黄怡晴1,邢  云1,刘顺治1,范世明2,蔡邦平3*,丘鹰昆1*   

  1. (1.厦门大学,福建 厦门 361105;2.福建中医药大学,福建 福州 350122;3.厦门园林植物园,福建 厦门 361003)
  • 收稿日期:2020-11-12 修回日期:2020-12-27 出版日期:2020-12-30 发布日期:2020-12-30
  • 通讯作者: 丘鹰昆
  • 基金资助:
    第四次全国中药资源普查项目(财社[2018]43号)

Two-dimensional Liquid Chromatography for the Identification of Euscaphis japonica var. wupingensis and E. japonica

HUANG Yi-qing1, XING Yun1, LIU Shun-zhi1, FAN Shi-ming2, CAI Bang-ping3*, QIU Ying-kun1*   

  1. (1.Fujian Provincial Key Laboratory of Innovative Drug Target Research, School of Pharmaceutical Sciences, Xiamen University, Xiamen 361105, Fujian China; 2.Fujian University of Traditional Chinese Medicine, Fuzhou 350122, Fujian China; 3.Xiamen Botanical Garden, Xiamen 361003, Fujian China)
  • Received:2020-11-12 Revised:2020-12-27 Online:2020-12-30 Published:2020-12-30
  • Contact: QIU Ying-kun

摘要: 运用在线二维液相色谱对野鸦椿(Euscaphis japonica)及其变种黄果野鸦椿(E. japonica var. wupingensis)的果皮化学成分进行对比分析。结果表明,黄果野鸦椿及其原变种的RPLC×RPLC二维色谱分析图谱较为相似,但仍有部分成分存在差异,进一步采用中心切割二维色谱定位了黄果野鸦椿及其原种的3个差异成分,为二者的化学鉴别提供借鉴。

关键词: 黄果野鸭椿, 二维液相色谱, RPLC×RPLC, 变种

Abstract: Online two-dimensional liquid chromatography was used for the comparative composition analysis of the pericarp of Euscaphis japonica var. wupingensis and E. japonica. The results showed that the two-dimensional RPLC×RPLC chromatograms were similar intraspecies, but some components were still different. In addition, heart-cutting two-dimensional chromatography was used to localize the three differential components between these two species. This experiment provided reference for the chemical differentiation of E. japonica var. wupingensis and E. japonica.

Key words: Euscaphis japonica var. wupingensis, two-dimensional liquid chromatography, RPLC×RPLC, variety

中图分类号: