旱柳与垂柳木质部细胞比较及CesA的表达分析

doi:10.3969/j.issn.1009-7791.2025.03.001

亚热带植物科学 ›› 2025, Vol. 54 ›› Issue (3): 239-248.DOI: 10.3969/j.issn.1009-7791.2025.03.001

• 研究论文 •

旱柳与垂柳木质部细胞比较及CesA的表达分析

王翌婷，郝晗睿，倪靖岚，陈艳红，张健，刘国元*

(南通大学生命科学学院 / 南通市观赏植物遗传育种重点实验室，江苏南通 226019)

收稿日期:2025-01-26 接受日期:2025-03-20 发布日期:2025-09-08
通讯作者: 刘国元
基金资助:
江苏省现代农业-碳达峰碳中和科技创新专项资金项目(BE2022420)；江苏省林业科技创新与推广项目(LYKJ[2023]02)；南通市自然科学基金项目(JC2024018)；江苏省研究生科研与实践创新计划项目(KYCX25_3683)；江苏省大学生创新创业训练计划项目(S202510304140)

Comparative Analysis of Xylem Cells in Salix matsudana and S. babylonica and Expression Analysis of Their CesA Genes

WANG Yi-ting, HAO Han-rui, NI Jing-lan, CHEN Yan-hong, ZHANG Jian , LIU Guo-yuan*

(School of Life Sciences, Nantong University / Nantong Key Laboratory of Landscape Plant Genetics and Breeding, Nantong 226019, Jiangsu China)

Received:2025-01-26 Accepted:2025-03-20 Published:2025-09-08
Contact: LIU Guo-yuan

摘要/Abstract

摘要： 以旱柳Salix matsudana和垂柳S. babylonica为试材，取不同位置枝条观察木质部细胞形态并测定其纤维素含量；在旱柳和垂柳全基因组测序基础上，鉴定纤维素合成酶基因CesA，并运用进化分析、染色体定位以及RNA-seq数据等多种手段，将结构变异位点与染色体定位进行联合分析。结果表明，垂柳枝条在相同位置木质部具有更薄的细胞壁和更低的纤维素含量。在旱柳和垂柳的全基因组序列中分别鉴定出25个和29 个CesA；该家族成员可分为10 个亚组，垂柳CesA基因不均等地分布在17条染色体上；CesA4/7/8在旱柳中显著高表达，推测其在纤维素合成中发挥着重要作用；染色体C18、C11和D11上的倒位改变了CesA7-3、CesA8-3和CesA8-4启动子区结构，染色体D2和D4的插入缺失影响CesA4-2和CesA8-2基因序列。因此，认为染色体结构变异影响了旱柳、垂柳中纤维素合酶基因CesA4/7/8表达，并改变纤维素含量与细胞壁厚度，最终造成旱柳与垂柳枝条形态的差异。

关键词: 旱柳；垂柳；纤维素；纤维素合酶基因；结构变异

Abstract: Taking Salix matsudana and S. babylonica as test materials, branches from different positions were sectioned and stained to determine their cellulose content. Based on the whole-genome sequencing of S. matsudana and S. babylonica, cellulose synthase genes were identified. A variety of approaches, including phylogenetic analysis, chromosome mapping, and RNA-seq data, were employed. Structural variation sites were also jointly analyzed with chromosome localization. The results indicate that branches of S. babylonica have thinner cell wall thickness and lower cellulose content at the same positions compared to S. matsudana. Based on the conserved domain features of cellulose synthase, we identified 25 and 29 cellulose synthase genes in the whole-genome sequencing results of S. matsudana and S. babylonica, respectively. Phylogenetic analysis indicated that the family members could be divided into 10 subgroups, and chromosome mapping showed that CesA genes in S. babylonica were unevenly distributed across 17 chromosomes. RNA-seq data analysis revealed that CesA4/7/8 were significantly highly expressed in S. matsudana, suggesting their important role in cellulose synthesis. Joint analysis of structural variation sites and gene localization found that inversions on chromosomes C18, C11, and D11 altered the structure of the promoter regions of CesA7-3, CesA8-3 and CesA8-4, while insertions and deletions on chromosomes D2 and D4 affected the gene sequences of CesA4-2 and CesA8-2. Chromosomal structural variations have impacted the expression of cellulose synthase genes CesA4/7/8 in S. matsudana and S. babylonica, altering the cellulose content and cell wall thickness. These changes ultimately lead to the differences in branch morphology between S. matsudana and S. babylonica.

Key words: Salix matsudana; Salix babylonica; cellulose; cellulose synthase genes; structural variatio

中图分类号:

Q943

王翌婷，郝晗睿，倪靖岚，陈艳红，张健，刘国元. 旱柳与垂柳木质部细胞比较及CesA的表达分析[J]. 亚热带植物科学, 2025, 54(3): 239-248.

WANG Yi-ting, HAO Han-rui, NI Jing-lan, CHEN Yan-hong, ZHANG Jian, LIU Guo-yuan. Comparative Analysis of Xylem Cells in Salix matsudana and S. babylonica and Expression Analysis of Their CesA Genes[J]. Subtropical Plant Science, 2025, 54(3): 239-248.

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旱柳与垂柳木质部细胞比较及CesA的表达分析

Comparative Analysis of Xylem Cells in Salix matsudana and S. babylonica and Expression Analysis of Their CesA Genes

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