亚热带植物科学 ›› 2025, Vol. 54 ›› Issue (6): 682-690.DOI: 10.3969/j.issn.1009-7791.2025.06.010

• 研究论文 • 上一篇    下一篇

尾水人工湿地根际真菌群落结构及功能分析

宋凤鸣,郭丹丹,莫  剑,杨  帆,王文明*   

  1. (湖南先导洋湖再生水有限公司,湖南 长沙 410208)
  • 收稿日期:2025-07-09 接受日期:2025-11-27 出版日期:2025-12-31 发布日期:2025-12-31
  • 通讯作者: 王文明
  • 基金资助:
    湖南省重点研发计划项目(2024AQ2005)

Structure and Function of Rhizosphere Fungal Community in Constructed Wetland with Tail Water

SONG Feng-ming, GUO Dan-dan, MO Jian, YANG Fan, WANG Wen-ming*   

  1. (Hunan Pilot Yanghu Reclaimed Water Co., Ltd., Changsha 410208, Hunan China)
  • Received:2025-07-09 Accepted:2025-11-27 Online:2025-12-31 Published:2025-12-31
  • Contact: WANG Wen-ming

摘要: 采集长沙市洋湖人工湿地不同工艺节点下冬夏两季黄菖蒲Iris pseudacorus、美人蕉Canna indica、香蒲Typha orientalis、芦苇Phragmites australis等植物根际沉积物,利用Illumina NovaSeq平台对真菌ITS区进行高通量测序,结合FUNGuild功能预测与冗余分析,探讨真菌群落结构、多样性及其与环境因子的关系。结果表明,优势真菌门为子囊菌门Ascomycota、壶菌门Chytridiomycota、毛霉门Mucoromycota和担子菌门Basidiomycota。毛霉门、子囊菌门、壶菌门和芽枝霉门Blastocladiomycota与总氮、有机质呈显著负相关,而捕虫霉门Zoopagomycota、担子菌门及未知类群(Unclassified fungi)等与总氮、有机质呈正相关。真菌多样性在二级湿地及夏季样本中更丰富。功能预测显示,真菌以腐生营养型、病理营养型和共生营养型为主,其中丛枝菌根真菌在芦苇根际中丰度较高,可增强植物抗逆性与磷吸收能力。研究结果为解析尾水人工湿地中植物-微生物协同净化机制提供理论依据。

关键词: 人工湿地, 沉积物, 根际真菌群落, 多样性

Abstract: Rhizosphere fungi play a crucial role in plant growth, nutrient cycling, and pollutant degradation within constructed wetland ecosystems. This study focused on the Yanghu Artificial Wetland in Changsha, collecting rhizosphere sediments from various treatment stages of plants such as Iris pseudacorus, Canna indica, Typha angustifolia, and Phragmites australis during winter and summer seasons. High-throughput sequencing of fungal ITS regions was conducted using the Illumina NovaSeq platform, combined with FUNGuild functional prediction and redundancy analysis to explore fungal community structure, diversity, and their relationship with environmental factors. Results revealed that the dominant fungal phyla were Ascomycota, Chytridiomycota, Mucoromycota, and Basidiomycota. Mucoromycota, Ascomycota, Chytridiomycota, and Blastocladiomycota exhibited significant negative correlations with total nitrogen and organic matter, while Zoopagomycota, Basidiomycota, and unclassified fungi showed positive correlations. Fungal diversity was higher in secondary wetlands and summer samples. Functional prediction indicated that fungi were primarily saprophytic, pathogenic, and symbiotic in nutrition type, with arbuscular mycorrhizal fungi being more abundant in the rhizosphere of P. australis, potentially enhancing plant stress resistance and phosphorus absorption capacity. This study provides a theoretical basis for the plant-microbe synergistic purification mechanism in tail water constructed wetlands.

Key words: constructed wetland, sediment, rhizosphere fungal community, diversity

中图分类号: