紫外线对紫球藻的生物学效应

亚热带植物科学 ›› 2003, Vol. 32 ›› Issue (01): 8-10,13.

紫外线对紫球藻的生物学效应

江贤章,陈小兰,王明兹,陈必链,施巧琴

福建师范大学生物工程学院, 福建福州 350007

收稿日期:2002-07-05 出版日期:2003-03-10 发布日期:2003-03-10
作者简介:江贤章(1980-),男,福建福州人,1999级本科生,微生物生化专业。
基金资助:
福建省教育厅资助项目

Biological effect of ultraviolet radiation on Porphyridium cruentum

JIANG Xian-zhang, CHEN Xiao-lan, WANG Ming-zi, CHEN Bi-lian, SHI Qiao-qin

Bioengineering College, Fujian Teacher's University, Fuzhou 350007, Fujian China

Received:2002-07-05 Online:2003-03-10 Published:2003-03-10

摘要/Abstract

摘要： 采用紫外线辐照紫球藻,处理剂量分别为10s、20s、30s、60s、90s、120s和180s。结果表明,10s剂量对紫球藻有促长作用,20s及30s对藻细胞生长和各项生理生化指标影响不显著,30s以上处理对其生长以及代谢产物的合成与分泌有抑制作用,且照射时间越长,抑制越强烈,紫球藻对紫外线的耐受极限为120s。结果还表明,高剂量(20~90s)照射能增加单细胞代谢产物含量;经紫外线辐照后的藻细胞第二代培养物在生长速度、叶绿素a含量、胞外多糖和β-胡萝卜素产量等与对照组相似。

关键词: 紫球藻, 紫外线, 生物学效应, 胞外多糖, &beta, -胡萝卜素

Abstract: Treated with ultraviolet (UV)radiation for 10s, 20s, 30s, 60s, 90s, 120s and 180s, respectively, the biological effects of UV rays on Porphyridium cruentum were studied. The results showed that the growth of P. cruentum was promoted when irradiated for 10s, the metabolism and growth of P. cruentum were inhibited under UV radiation for more than 30s and the more the radiation time was treated, the more inhibited effects were found. The mortality of P. cruentum was 100% when treated with UV radiation for more than 120s. The results also showed that high dose of radiation (20-90s)improved the metabolic products of single cell. The second generation of irradiated cell was similar to that of initial cell (CK), including the rate of growth, the contents of chlorophyll a, extra cellular polysaccharides and β-carotene.

Key words: Porphyridium cruentum, ultraviolet rays, biological effect, extra cellular polysaccharide, β-carotene

中图分类号:

江贤章,陈小兰,王明兹,陈必链,施巧琴. 紫外线对紫球藻的生物学效应[J]. 亚热带植物科学, 2003, 32(01): 8-10,13.

JIANG Xian-zhang, CHEN Xiao-lan, WANG Ming-zi, CHEN Bi-lian, SHI Qiao-qin. Biological effect of ultraviolet radiation on Porphyridium cruentum[J]. Subtropical Plant Science, 2003, 32(01): 8-10,13.

[1]	罗恩锡，李真琴，洪克前，宋康华，梁永康，王俊宁. 短波紫外线照射对‘金都’火龙果采后保鲜的影响[J]. 亚热带植物科学, 2021, 50(05): 353-359.
[2]	钟娴,江琳玉,潘东明,潘腾飞,卞阿娜,. 漳州水仙ZDS 基因cDNA克隆及其表达分析[J]. 亚热带植物科学, 2013, 42(02): 97-103.
[3]	刘育梅,黄维南. 羊奶果果实色素的初步鉴定[J]. 亚热带植物科学, 2011, 40(04): 63-65.
[4]	刘梅,陈必链,陈伟平. 超声波降解紫球藻胞外多糖研究[J]. 亚热带植物科学, 2008, 37(01): 48-50.
[5]	曾秀丽,张光伦,李春燕,罗楠,胡强. 三个脐橙品种果实主要细胞壁酶动态变化研究[J]. 亚热带植物科学, 2006, 35(02): 12-16.
[6]	叶锦林,王明兹. 紫球藻及其多糖抗菌性能初探[J]. 亚热带植物科学, 2004, 33(03): 31-33.
[7]	王明兹,施巧琴,郑梅清,吴松刚. 紫球藻的培养与利用[J]. 亚热带植物科学, 2001, 30(02): 66-69.
[8]	黄曦,陈惠民. 蜗牛酶对原生质体游离的影响[J]. 亚热带植物科学, 1993, 22(02): 1-6.
[9]	林耀辉,许文江,刘新民,沈育芬. 灵芝胞外多糖的发酵条件及其组份的初步研究[J]. 亚热带植物科学, 1992, 21(02): 18-23.