关键词: 红花桑寄生, AB-8大孔吸附树脂, 总黄酮, 吸附分离, 影响因子

Abstract: Influencing factors of static adsorption and dynamic desorption by AB-8 macroporous resins for total flavoniods from Scurrula parasitica were studied, and effects of concentration of sample of extract solution and pH value, surroundings temperature and oscillatory rate on static adsorption capacity, and effects of ethanol concentration on dynamic elution were systematically analyzed.The results showed that concentration of sample and pH value of extract solution had significantly effect on resin absorption properties.The technological conditions of adsorption and desorption for total flavonoids were as follows:the concentration of extract 1.2~2.0 mg/ml;pH 3.0~4.0;the oscillatory rate 160r/min;room temperature;adsorption time 2~3 h;the eluting regent 50% ethanol (5 folds the volume of the resin);eluting rate 1.5 ml/min.Under the optimal conditions, the maximum adsorbing capacity of AB-8 resin for total flavoniods was 29.0 mg/g, with the dynamic elution ratio of 95.0%, the purity of flavonoids obtained was 46.0% and yield 5.5% as well.

Key words: Scurrula parasitica, AB-8 macroporous resin, total flavonoids, adsorption and separation, influencing factors