辣椒子叶再生体系的建立

亚热带植物科学 ›› 2007, Vol. 36 ›› Issue (03): 37-39,42.

辣椒子叶再生体系的建立

敬国兴^1,2,李伏钦¹,陈永双¹,曾富华^1,2,卢向阳²

1. 湛江师范学院生命科学与技术学院, 广东湛江 524048;
2. 湖南农业大学生化与发酵工程实验室, 湖南长沙 410128

收稿日期:2007-04-11 出版日期:2007-09-10 发布日期:2007-09-10
作者简介:敬国兴(1981-),男,湖南常德人,硕士研究生,从事分子生物学研究。
基金资助:
广东省科技计划项目(Z002A2070402);广东省自然科学基金(5011730)

Establishment of Regeneration System of Pepper Cotyledon

JING Guo-xing^1,2, LI Fu-qin¹, CHEN Yong-shuang¹, ZENG Fu-hua^1,2, LU Xiang-yang²

1. School of Life Science & Technology, Zhanjiang Normal University, Zhanjiang 524048, Guangdong China;
2. Laboratory of Biochemistry and Fermentation Engineering, Hunan Agricultural University, Changsha 410128, Hunan China

Received:2007-04-11 Online:2007-09-10 Published:2007-09-10

摘要/Abstract

摘要： 以保加利亚尖椒和赤研6号椒子叶为试材,探讨植物生长调节剂及其组合(LC与IAA)对不定芽诱导分化及伸长的影响。结果表明,LC 1.0 mg/L+IAA 0.1 mg/L和LC 1.0 mg/L+IAA 1.0 mg/L均有利于保加利亚尖椒不定芽的分化,分化率均达100%,优胜芽率分别为83.3%和66.7%;LC 0.1 mg/L+IAA 0.2 mg/L有利于保加利亚尖椒不定芽的伸长,伸长率达78.5%,而LC 0.2 mg/L+IAA 0.6 mg/L有利于赤研6号椒不定芽的伸长,伸长率达58.5%;1/2 MS+IAA 1.0 mg/L+NAA 0.5 mg/L有利于不定芽的生根,保加利亚尖椒和赤研6号椒生根率分别为100%和85%。

关键词: 辣椒, 子叶, 再生

Abstract: Effects of plant regulators on adventitious buds differentiation and elongation of Bulgaria pointed pepper and chiyan No.6 were studied in the report.The results showed that the best combinations for the adventitious buds differentiation of Bulgaria pointed pepper and chiyan No.6 were LC 1.0 mg/L+IAA 0.1 mg/L and LC 1.0 mg/L+IAA 1.0 mg/L, respectively, with which the differentiation rates were all 100%, the rates of superior buds were 83.3% and 66.7%, respectively.Best combinations for elongation of adventitious buds of Bulgaria pointed pepper and chiyan No.6 were LC 0.1 mg/L+IAA 0.2 mg/L and LC 0.2 mg/L+IAA 0.6 mg/L, respectively, the elongation rates were 78.5% and 58.5%, respectively.The optimum rooting medium was 1/2 MS+IAA 1.0 mg/L+NAA 0.5 mg/L, and the average rooting rates were 100% and 85%, respectively.

Key words: Capsicum annuum, cotyledon, regeneration

中图分类号:

Q943.1

敬国兴,李伏钦,陈永双,曾富华,卢向阳. 辣椒子叶再生体系的建立[J]. 亚热带植物科学, 2007, 36(03): 37-39,42.

JING Guo-xing, LI Fu-qin, CHEN Yong-shuang, ZENG Fu-hua, LU Xiang-yang. Establishment of Regeneration System of Pepper Cotyledon[J]. Subtropical Plant Science, 2007, 36(03): 37-39,42.

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