亚热带植物科学 ›› 2008, Vol. 37 ›› Issue (02): 38-40.

• 研究报告 • 上一篇    下一篇

秋水仙素诱导兰属‘素心黄’多倍体的方法研究

邓樱,周晔,陈继敏   

  1. 珠海市农业科学研究中心, 广东 珠海 519070
  • 收稿日期:2007-12-05 出版日期:2008-06-10 发布日期:2008-06-10
  • 作者简介:邓樱(1971-),男,湖南邵阳人,农艺师,硕士,从事花卉组织培养及育种研究。

Methods of Polyploid Induction of Cymbidium with Colchicine

DENG Ying, ZHOU Ye, CHEN Ji-min   

  1. Zhuhai Research Center of Agricultural Sciences, Zhuhai 519070, Guangdong China
  • Received:2007-12-05 Online:2008-06-10 Published:2008-06-10

摘要: 采用继代培养法和侧芽萌动培养法比较秋水仙素诱导‘素心黄’多倍体的效果。结果表明,继代培养法加倍效果较好,其诱导率为16.7%;细胞学鉴定表明,该方法诱导培育的植株具有多倍体表型特征,其体细胞染色体数2n=80,未见嵌合现象,而对照2n=40。

关键词: 兰属&lsquo, 素心黄&rsquo, 原球茎, 根尖, 秋水仙素, 多倍体诱导

Abstract: The methods of polyploid induction of Cymbidium by colchicine were studied in this paper. The results showed that the methods of multiplicating PLBs gave a best result for polyploid induction, and about 16.7% protocorms developed into polyploidies. Cytological observation showed that the plantlets had the characteristics of polyploid phenotype and the chromosome number was 2n=80, but no mosaic phenomenon were observed, and the chromosome number of the control (no treated with colchicines) was 2n=40.

Key words: Cymbidium, protocorm, root tip, colchicine, polyploid induction

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