硒对三裂叶野葛毛状根生长及抗氧化酶活性的影响

亚热带植物科学 ›› 2009, Vol. 38 ›› Issue (01): 23-25,30.

硒对三裂叶野葛毛状根生长及抗氧化酶活性的影响

何含杰^1,2,施和平²

1. 商丘师范学院生命科学系, 河南商丘 476000;
2. 华南师范大学生命科学学院, 广东省植物发育生物工程重点实验室, 广东广州 510631

收稿日期:2008-10-11 出版日期:2009-03-10 发布日期:2009-03-10
作者简介:何含杰(1979-),男,河南商丘人,硕士,讲师,从事药用植物细胞工程研究。

Effects of Selenium on Growth and Anti-oxidative Enzyme Activities in Pueraria phaseoloides Hairy Roots

HE Han-jie^1,2, SHI He-ping²

1. Department of Life Science, Shangqiu Normal College, Shangqiu 476000, Henan China;
2. Guangdong Key Lab of Biotechnology for Plant Development, College of Life Sciences, South China Normal University, Guangzhou 510631, Guangdong China

Received:2008-10-11 Online:2009-03-10 Published:2009-03-10

摘要/Abstract

摘要： 本文研究硒(Se)对三裂叶野葛毛状根生长及抗氧化酶活性的影响。结果表明,低浓度亚硒酸钠(0~0.5 mg/L)可促进毛状根生长及异黄酮的生物合成,而高浓度亚硒酸钠抑制其生长和异黄酮的积累,且浓度愈高抑制作用愈强。此外,硒可促进可溶性蛋白的生物合成,亚硒酸钠浓度愈高促进作用愈明显;硒还可降低毛状根中SOD活性,提高POD活性,降低MDA含量。说明硒对三裂叶野葛毛状根生长和抗氧化酶活性有重要的调节作用。

关键词: 亚硒酸钠, 三裂叶野葛, 毛状根, 异黄酮, 抗氧化酶

Abstract: Effects of selenium (Se) on the growth and anti-oxidative enzyme activities of Pueraria phaseoloides hairy roots were investigated.The results showed that low concentration of Se (0~0.5 mg/L) promoted the growth and isoflavones biosynthesis of hairy roots,and high concentration of Se inhibited the growth and isoflavones accumulation,and the inhibition effects increased with the concentration of Se.In addition,Se also promoted the biosynthesis of soluble protein,and the effects increased with the concentration of.Meanwhile,Se decreased the activity of SOD and content of MDA,enhanced the activity of POD.It was suggested that Se play an important regulative role in the growth and antioxidant enzyme activities of P. phaseoloides hairy roots.

Key words: selenium, Pueraria phaseoloides, hairy roots, isoflavones, antioxidant enzyme

中图分类号:

Q945.12

何含杰,施和平. 硒对三裂叶野葛毛状根生长及抗氧化酶活性的影响[J]. 亚热带植物科学, 2009, 38(01): 23-25,30.

HE Han-jie, SHI He-ping. Effects of Selenium on Growth and Anti-oxidative Enzyme Activities in Pueraria phaseoloides Hairy Roots[J]. Subtropical Plant Science, 2009, 38(01): 23-25,30.

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