NRRB在水稻应答高盐和干旱胁迫中的功能解析

doi:10.3969/j.issn.1009-7791.2015.04.001

亚热带植物科学 ›› 2015, Vol. 44 ›› Issue (04): 267-273.DOI: 10.3969/j.issn.1009-7791.2015.04.001

• 植物生理生化与分子生物学 • 下一篇

NRRB在水稻应答高盐和干旱胁迫中的功能解析

张玉霞,周丹,吴敏亮,李燕,杨钰莹,陈亮

(厦门大学生命科学学院，厦门市植物遗传重点实验室，福建厦门 361102)

收稿日期:2015-10-23 修回日期:2016-01-15 出版日期:2015-12-10 发布日期:2015-12-10
通讯作者: 陈亮
作者简介:张玉霞，博士研究生，从事植物基因工程研究。
基金资助:
国家重点基础研究发展计划(973)前期研究专项(No. 2011ZX08001-001)

Functional Analysis of NRRB in Rice Response to Salt and Drought Stresses

ZHANG Yu-xia,ZHOU Dan,WU Min-liang,LI Yan,YANG Yu-ying,CHEN Liang

(Xiamen Key Laboratory for Plant Genetics, School of Life Sciences, Xiamen University, Xiamen 361102, Fujian China)

Received:2015-10-23 Revised:2016-01-15 Online:2015-12-10 Published:2015-12-10
Contact: CHEN Liang

摘要/Abstract

摘要： 为研究NRRB在水稻抗逆反应中的作用，通过重叠延伸PCR扩增NRRB基因编码区，构建超量表达载体，并转化水稻愈伤组织获得超量表达转基因水稻植株。鉴定结果表明，该基因已被整合到水稻基因组中，并实现超量表达；同时构建了抑制表达载体，获得转基因株系，PCR检测结果证实NRRB基因在转基因水稻中受到明显抑制。对T1代转基因植株进行抗旱性、耐盐性分析，结果显示，超量表达NRRB基因增强了转基因水稻对干旱的抗性，抑制表达NRRB基因的转基因水稻对干旱的敏感性增强，表明NRRB正调控水稻对干旱的抗性；耐盐性分析表明，NRRB基因的抑制表达降低了植株对盐的敏感性。

关键词: NRRB, 超量表达载体, RNAi表达载体, 转基因水稻

Abstract: To explore the role of NRRB protein in rice resistance to abiotic stress, we amplified gene coding region by overlap extension PCR, constructed over-expression vector, and transformed into rice callus then obtained transgenic lines. The identification results showed that NRRB gene had been integrated into the rice genome, and was overexpressed in positive transgenic plants. Meanwhile, interference expression vector was constructed and transgenic lines were obtained, PCR result confirmed that the expression of NRRB was notably downregulated. The research on drought resistance in the T1 generation of transgenic plants indicated that overexpressing NRRB improved resistance of the transgenic plants to drought, and suppressing its expression enhanced susceptibility of the transgenic plants to drought, indicating that NRRB positively regulates resistance of rice to drought. The results of salt tolerance showed that suppressing NRRB reduced the susceptibility of the transgenic plants to salt.

Key words: NRRB, over-expression vector, RNAi-expression vector, transgenic rice

中图分类号:

Q943.2

张玉霞,周丹,吴敏亮,李燕,杨钰莹,陈亮. NRRB在水稻应答高盐和干旱胁迫中的功能解析[J]. 亚热带植物科学, 2015, 44(04): 267-273.

ZHANG Yu-xia,ZHOU Dan,WU Min-liang,LI Yan,YANG Yu-ying,CHEN Liang. Functional Analysis of NRRB in Rice Response to Salt and Drought Stresses[J]. Subtropical Plant Science, 2015, 44(04): 267-273.

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NRRB在水稻应答高盐和干旱胁迫中的功能解析

Functional Analysis of NRRB in Rice Response to Salt and Drought Stresses

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