›› 2016, Vol. 45 ›› Issue (01): 17-20.DOI: 10.3969/j.issn.1009-7791.2016.01.004

• 植物生理生化与分子生物学 • 上一篇    下一篇

独一味总黄酮提取工艺优化

程力惠   

  1. (广东药学院,广东 广州 510627)
  • 收稿日期:2015-12-30 修回日期:2016-04-17 出版日期:2016-03-10 发布日期:2016-03-10
  • 通讯作者: 程力惠
  • 作者简介:程力惠,硕士,副教授,从事天然药物研究。
  • 基金资助:
    广东省科技计划项目(2007B030702005)

Optimization of Extraction Process of Total Flavonoids from Lamiophlomis rotata

CHENG Li-hui   

  1. (Guangdong Pharmic University, Guangzhou 510627, Guangdong China)
  • Received:2015-12-30 Revised:2016-04-17 Online:2016-03-10 Published:2016-03-10
  • Contact: CHENG Li-hui

摘要: 以独一味Lamiophlomis rotata总黄酮提取率为考察指标,比较乙醇回流法、水煎法、超声法三种提取方法优劣,并通过L9(34)正交试验优选出最佳提取条件。结果显示,乙醇回流法的黄酮提取率显著高于其他两种方法,其最佳提取工艺为用80%乙醇溶液回流提取3次,料液比为1∶50,提取时间25 min。

关键词: 独一味, 总黄酮, 提取工艺

Abstract: Taken the extraction rate of total flavonoids from Lamiophlomis rotata as index, extraction methods, the alcohol concentration, solid-liquid ratio, extraction duration and extraction times were compared. Based on signal factor results, orthogonal test factors and levels were determined to optimize extraction condition by L9(34). The optimum extraction process were as follows: extraction solvent was 80% ethanol, solid-liquid ratio 1∶50, extraction time was 25 min for 3 times. The extraction process was simple and reasonable, which could effectively improve the extraction rate of total flavonoids from L. rotata.

Key words: Lamiophlomis rotata, total flavonoid, extraction

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