亚热带植物科学 ›› 2018, Vol. 47 ›› Issue (02): 124-127.DOI: 10.3969/j.issn.1009-7791.2018.02.006

• 植物生理生化与分子生物学 • 上一篇    下一篇

秋水仙素对洋葱根尖细胞染色体结构变异的影响

刘菲婷,李沁,李悦,郭海滨,李楠   

  1. (华南农业大学公共基础课实验教学中心,广东 广州 510642)
  • 收稿日期:2018-04-03 修回日期:2018-04-17 出版日期:2018-06-30 发布日期:2018-06-30
  • 通讯作者: 李楠
  • 作者简介:刘菲婷,本科生,花卉与景观设计专业。
  • 基金资助:
    华南农业大学大学生创新创业训练计划项目(201610564506);华南农业大学教育教学改革与研究项目(JG17093)

Effect of Colchicine on Chromosome Structure Variation of Onion Root Tip Cells

LIU Fei-ting, LI Qin, LI Yue, GUO Hai-bin, LI Nan   

  1. (Center of Experimental Teaching for Common Basic Courses, South China Agricultural University, Guangzhou 510642, Guangdong China)
  • Received:2018-04-03 Revised:2018-04-17 Online:2018-06-30 Published:2018-06-30
  • Contact: LI Nan

摘要: 用0.05%和0.1%秋水仙素溶液对洋葱Allium cepa根尖进行控时处理,研究不同秋水仙素浓度和不同处理时间对洋葱根尖细胞有丝分裂畸变的影响。结果显示,秋水仙素有促进细胞有丝分裂染色体停滞在中期的能力;不同固定时间对洋葱根尖细胞有丝分裂有影响;秋水仙素对洋葱根尖细胞染色体有畸变效应,诱导产生畸变的能力受溶液浓度和处理时间的影响。

关键词: 秋水仙素, 结构变异, 洋葱

Abstract: Treated with 0.05% and 0.1% colchicine solution by controlling time, effects of different colchicine concentrations and different treatment times on onion root tip distortion were researched. The results showed that colchicine had the ability to promote cell mitosis and stagnate cell chromosome in medium term. Different fixation times had effects on the mitosis of onion root tip cells. Colchicine had a distorted effect on onion root tip cells, and the ability to induce distortion could be influenced by colchicine solution concentration and treatment time.

Key words: colchicine, structure variation, onion

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