白毫银针茶水提取物对LO2细胞脂质积累的影响及其机理研究

doi:10.3969/j.issn.1009-7791.2018.01.001

亚热带植物科学 ›› 2018, Vol. 47 ›› Issue (1): 1-7.DOI: 10.3969/j.issn.1009-7791.2018.01.001

• 植物生理生化与分子生物学 • 下一篇

白毫银针茶水提取物对LO2细胞脂质积累的影响及其机理研究

林秋香,陈曦,谢剑威,刘乾刚

(福建农林大学园艺学院/茶学福建省普通高校重点实验室，福建福州 350002)

收稿日期:2018-01-29 修回日期:2018-02-07 出版日期:2018-03-30 发布日期:2018-03-30
通讯作者: 刘乾刚
作者简介:林秋香，硕士研究生，从事茶叶深加工与资源利用研究。
基金资助:
国家标准化委员会、福建省质检局项目“白茶国际标准研究”(KY0020088)

Effect of Silver Needle Tea Extract on Lipid Accumulation in LO2 Cells and Its Mechanism

LIN Qiu-xiang,CHEN Xi,XIE Jian-wei,LIU Qian-gang

(College of Horticulture, Fujian Agriculture and Forestry University/Key Laboratory of Tea Science in Universities of Fujian Province, Fuzhou 350002, Fujian China)

Received:2018-01-29 Revised:2018-02-07 Online:2018-03-30 Published:2018-03-30
Contact: LIU Qian-gang

摘要/Abstract

摘要： 以白毫银针水提取物为供试制剂，探讨其对LO2细胞脂质积累的影响及作用机理。通过油酸处理建立非酒精性脂肪肝细胞模型，分别检测白毫银针水提取物治疗组、预防组、辛伐他汀组、自然恢复组、模型组LO2细胞的甘油三脂(TG)和丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性、脂质积累，并测定细胞内SREBP-1c、FAS和CPT1A mRNA表达变化。结果表明，1 mmol·L-1油酸处理24 h为建立非酒精性脂肪肝细胞模型的最理想条件，模型组细胞内TG和MDA含量高于正常组，但SOD活性低于正常组；水提取物处理各组别中，治疗组1 mg·mL-1白毫银针制剂处理的细胞MDA含量最低，脂肪颗粒最少，SREBP-1c和FAS相对表达量最低，但CPT1A相对表达量和SOD活性最高，为本实验非酒精性脂肪肝防治的最优处理。1 mg·mL-1白毫银针制剂能显著降低LO2细胞的脂质积累，其机制可能与抑制SREBP-1c及其下游的FAS基因表达，减少TG合成，增加CPT1A基因表达，加速TG分解等因素有关。

关键词: 白毫银针, LO2细胞, 脂质积累, 非酒精性脂肪肝, 预防

Abstract: In this paper, the aqueous extract of silver needle tea was used as the experimental preparation for study of its effect and mechanism on the lipid accumulation in LO2 cells. The cell model of non-alcoholic fatty liver was established by the treatment of oleic acid, and the therapeutic group, preventive group, simvastatin group and natural recovery group were arranged and respectively applied to the above experimental preparations and detected for triglycerides (TG) and malondialdehyde (MDA) content, superoxide dismutase (SOD) activity, lipid accumulation and SREBP-1c, FAS and CPT1A mRNA expressions in LO2 cells. In this investigation, 1 mmol·L-1 oleic acid treatment for 24 h was the optimal condition for establishment of non-alcoholic fatty liver cell model, the contents of TG and MDA in the model group were higher than that in the normal group, while the SOD activity was lower when compared with the normal group, and among the groups the treatment of 1 mg·mL-1 aqueous extract in therapeutic group showed the lowest MDA content, the lowest lipid accumulation，the lowest relative expression of SREBP-1c and FAS，but the highest SOD activity and the highest relative expression of CPT1A of LO2 cells, and was regarded as the optimal aqueous preparation for prevent and treat the non-alcoholic fatty liver. The lipid accumulation of LO2 cells was significantly reduced by the treatment of the 1 mg·mL-1 aqueous extract, which may be related to the inhibition of SREBP-1c and the successive FAS gene expression, the decrease of TG synthesis, the acceleration of CPT1A gene expression and decomposition of TG, etc.

Key words: silver needle tea, LO2 cells, Lipid accumulation, non-alcoholic fatty liver disease, prevention

中图分类号:

TS272

林秋香,陈曦,谢剑威,刘乾刚. 白毫银针茶水提取物对LO2细胞脂质积累的影响及其机理研究[J]. 亚热带植物科学, 2018, 47(1): 1-7.

LIN Qiu-xiang,CHEN Xi,XIE Jian-wei,LIU Qian-gang. Effect of Silver Needle Tea Extract on Lipid Accumulation in LO2 Cells and Its Mechanism[J]. Subtropical Plant Science, 2018, 47(1): 1-7.

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白毫银针茶水提取物对LO2细胞脂质积累的影响及其机理研究

Effect of Silver Needle Tea Extract on Lipid Accumulation in LO2 Cells and Its Mechanism

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