›› 2018, Vol. 47 ›› Issue (1): 77-80.DOI: 10.3969/j.issn.1009-7791.2018.01.016

• 植物栽培与组培 • 上一篇    下一篇

盘龙参组织培养与快繁研究

吕亚凤,郭德章,魏广彪   

  1. (福州市农业科学研究所,福建 福州350018)
  • 收稿日期:2017-12-12 出版日期:2018-03-30 发布日期:2018-03-30
  • 通讯作者: 吕亚凤
  • 作者简介:吕亚凤,硕士,助理研究员,从事园艺植物生物技术研究。

Tissue Culture and Rapid Propagation of Spiranthes sinensis

L? Ya-feng,GUO De-zhang,WEI Guang-biao   

  1. (Fuzhou Institute of Agricultural Sciences, Fuzhou 350018, Fujian China)
  • Received:2017-12-12 Online:2018-03-30 Published:2018-03-30
  • Contact: L? Ya-feng

摘要: 以野生花卉植物盘龙参Spiranthes sinensis的芽、花序为外植体,探讨不同消毒处理及植物生长调节剂对盘龙参组织培养的影响,建立组织培养快繁体系。结果显示,以0.1%升汞消毒6 min处理效果较好;丛生芽形成、花序愈伤组织诱导最适合的培养基组合为1/2MS + 6-BA 2.5 mg·L-1 + NAA 0.25 mg·L-1;最佳增殖培养基组合为1/2MS + 6-BA 2.0 mg·L-1 + NAA 0.75 mg·L-1;最佳生根培养基为1/2MS + NAA 1.25 mg·L-1。

关键词: 盘龙参, 组织培养, 快繁, 芽, 花序

Abstract: The effects of different disinfection treatment and the combinations of plant growth regulators on Spiranthes sinensis tissue culture with bud and inflorescence as explants were studied, and the rapid propagation system for tissue culture was established. The results indicated that the appropriate method for disinfection treatment on explants was in 0.1% mercuric chloride for 6 min. The optimal induction medium for multiple buds formation and callus induction was 1/2MS + 6-BA 2.5 mg稬-1 + NAA 0.25 mg稬-1. The optimum medium for proliferation was 1/2MS + 6-BA 2.0 mg稬-1 + NAA 0.75 mg稬-1. The optimum rooting medium was 1/2MS + NAA 1.25 mg稬-1.

Key words: Spiranthes sinensis, tissue culture, rapid propagation, bud, inflorescence

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