HPLC法测定小叶山葡萄叶中白藜芦醇含量

doi:10.3969/j.issn.1009-7791.2019.04.005

亚热带植物科学 ›› 2019, Vol. 48 ›› Issue (04): 334-338.DOI: 10.3969/j.issn.1009-7791.2019.04.005

HPLC法测定小叶山葡萄叶中白藜芦醇含量

何恩铭，田淼，沈瑞池，任育军，汪文华

(1.福建省亚热带植物研究所／福建省亚热带植物生理生化重点实验室，福建厦门 361006；2.福建农林大学生命科学学院分子细胞与系统生物学中心，福建福州 350002)

收稿日期:2019-02-28 修回日期:2019-10-23 出版日期:2019-12-30 发布日期:2019-12-30
通讯作者: 汪文华
作者简介:何恩铭，博士，副研究员，从事植物资源开发利用研究。
基金资助:
厦门市科技计划项目(3502Z20151245)；厦门市重大科技创新平台项目(3502Z20131004)

Determination of Resveratrol in Vitis thunbergii var. taiwaniana Leaves by HPLC

HE En-ming, TIAN Miao, SHEN Rui-chi, REN Yu-jun, WANG Wen-hua

(1.Fujian Institute of Subtropical Botany／Fujian Provincial Key Laboratory of Subtropical Plant Physiology and Biochemistry, Xiamen 361006, Fujian China；2.Center for Molecular Cell and Systems Biology, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian China)

Received:2019-02-28 Revised:2019-10-23 Online:2019-12-30 Published:2019-12-30
Contact: WANG Wen-hua

摘要/Abstract

摘要： 建立一种高效液相色谱法(HPLC)测定小叶山葡萄Vitis thunbergii var. taiwaniana叶中白藜芦醇含量的检测方法。Waters高效液相色谱仪：Hypersil C18色谱柱(250 mm × 4.6 mm，5 μm)；流动相：甲醇-水(72:28)；流速：1 mL·min-1；检测波长306 nm。结果表明，在上述色谱条件下，白藜芦醇含量在10～200 μg·mL-1范围内线性关系良好，相关系数r为0.9999。精密度、重现性、稳定性的RSD(n=5)分别为0.77%、0.41%、0.21%；平均加标回收率为99.92 %，相对标准偏差为0.39 %。该方法准确、灵敏、可靠，可用于白藜芦醇的定量和定性分析。

关键词: HPLC, 小叶山葡萄, 白藜芦醇, 测定

Abstract: A high-performance liquid chromatography method for determination of resveratrol was developed. The determination of resveratrol was performed on a Hypersil C18 column (250 mm × 4.6 mm, 5 μm). The mobile phase was methanol-water (72:28), and the flow rate was 1 mL·min-1. The detector was photodiode array detector and the variable wavelength detector was set at 306 nm. In the above chromatographic conditions, good linear was observed in the linear range of 10～200 μg·mL-1, and the correlation coefficient (r) was 0.9999. The relative standard deviation (RSD) of the precision, reproducibility and stability were 0.77%, 0.41% and 0.21%, respectively. The average recovery of standard addition was 99.92% with RSD of 0.39%. The method is sensitive, reliable, accurate, and could be used for the determination of resveratrol.

Key words: HPLC, Vitis thunbergii var. taiwaniana, resveratrol, determination

中图分类号:

R927.2

何恩铭，田淼，沈瑞池，任育军，汪文华. HPLC法测定小叶山葡萄叶中白藜芦醇含量[J]. 亚热带植物科学, 2019, 48(04): 334-338.

HE En-ming, TIAN Miao, SHEN Rui-chi, REN Yu-jun, WANG Wen-hua. Determination of Resveratrol in Vitis thunbergii var. taiwaniana Leaves by HPLC[J]. Subtropical Plant Science, 2019, 48(04): 334-338.

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HPLC法测定小叶山葡萄叶中白藜芦醇含量

Determination of Resveratrol in Vitis thunbergii var. taiwaniana Leaves by HPLC

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